| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SERPINB1 (100-379) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SERPINB1 |
| Gene ID: |
1992 |
| Uniprot ID: |
ILEU_HUMAN |
| Immunogen Region: |
100-379 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 100-379 of human SERPINB1 (NP_109591.1). |
| Immunogen Sequence: |
FLPEFLVSTQKTYGADLASV DFQHASEDARKTINQWVKGQ TEGKIPELLASGMVDNMTKL VLVNAIYFKGNWKDKFMKEA TTNAPFRLNKKDRKTVKMMY QKKKFAYGYIEDLKCRVLEL PYQGEELSMVILLPDDIEDE STGLKKIEEQLTLEKLHEWT KPENLDFIEVNVSLPRFKLE ESYTLNSDLARLGVQDLFNS SKADLSGMSGARDIFISKIV HKSFVEVNEEGTEAAAATA |
| Tissue Specificity | In human bone marrow, present in all CD45+ populations. Expression levels are highest in the neutrophil lineage, intermediate in monocytic, and lowest in lymphocytic lineage. Within the neutrophil lineage, expression is highest in promyelocytes. |
| Function | Neutrophil serine protease inhibitor that plays an essential role in the regulation of the innate immune response, inflammation and cellular homeostasis. Acts primarily to protect the cell from proteases released in the cytoplasm during stress or infection. These proteases are important in killing microbes but when released from granules, these potent enzymes also destroy host proteins and contribute to mortality. Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Acts also as a potent intracellular inhibitor of GZMH by directly blocking its proteolytic activity. During inflammation, limits the activity of inflammatory caspases CASP1, CASP4 and CASP5 by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation. When secreted, promotes the proliferation of beta-cells via its protease inhibitory function. |
| Protein Name | Leukocyte Elastase InhibitorLeiMonocyte/Neutrophil Elastase InhibitorEiM/NeiPeptidase Inhibitor 2Pi-2Serpin B1 |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | SecretedCytoplasmCytolytic GranuleEarly Endosome |
| Alternative Antibody Names | Anti-Leukocyte Elastase Inhibitor antibodyAnti-Lei antibodyAnti-Monocyte/Neutrophil Elastase Inhibitor antibodyAnti-Ei antibodyAnti-M/Nei antibodyAnti-Peptidase Inhibitor 2 antibodyAnti-Pi-2 antibodyAnti-Serpin B1 antibodyAnti-SERPINB1 antibodyAnti-ELANH2 antibodyAnti-MNEI antibodyAnti-PI2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
