| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SERPINA3 |
| Gene ID: | 12 |
| Uniprot ID: | AACT_HUMAN |
| Immunogen Region: | 24-423 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 24-423 of human Alpha-1 Antichymotrypsin (SERPINA3) (NP_001076.2). |
| Immunogen Sequence: | HPNSPLDEENLTQENQDRGT HVDLGLASANVDFAFSLYKQ LVLKAPDKNVIFSPLSISTA LAFLSLGAHNTTLTEILKGL KFNLTETSEAEIHQSFQHLL RTLNQSSDELQLSMGNAMFV KEQLSLLDRFTEDAKRLYGS EAFATDFQDSAAAKKLINDY VKNGTRGKITDLIKDLDSQT MMVLVNYIFFKAKWEMPFDP QDTHQSRFYLSKKKWVMVPM MSLHHLTIPYFRDEELSCT |
| Tissue Specificity | Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains. |
| Post Translational Modifications | N- and O-glycosylated. |
| Function | Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. |
| Protein Name | Alpha-1-AntichymotrypsinActCell Growth-Inhibiting Gene 24/25 ProteinSerpin A3 Cleaved Into - Alpha-1-Antichymotrypsin His-Pro-Less |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-6798695 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Alpha-1-Antichymotrypsin antibodyAnti-Act antibodyAnti-Cell Growth-Inhibiting Gene 24/25 Protein antibodyAnti-Serpin A3 Cleaved Into - Alpha-1-Antichymotrypsin His-Pro-Less antibodyAnti-SERPINA3 antibodyAnti-AACT antibodyAnti-GIG24 antibodyAnti-GIG25 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and SERPINA3 knockdown (KD) Hep G2 cells using [KD Validated] SERPINA3 Rabbit pAb (STJ11107100) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit (](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/186296/408037/STJ11107100_1__46493.1744894472.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SERPINA3 knockdown (KD) Hep G2 cells using [KD Validated] SERPINA3 Rabbit pAb (STJ11107100) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit (")
