• Western blot analysis of extracts of various cell lines, using Septin 9 antibody (STJ111369) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Confocal immunofluorescence analysis of U2OS cells using Septin 9 Polyclonal Antibody (STJ111369) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Confocal immunofluorescence analysis of U2OS cells using Septin 9 Polyclonal Antibody (STJ111369) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using Septin 9 antibody (STJ111369) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SEPT9 antibody (1-110) (STJ111369)

SKU:
STJ111369

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SEPTIN9
Gene ID: 10801
Uniprot ID: SEPT9_HUMAN
Immunogen Region: 1-110
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human Septin 9 (NP_001106964.1).
Immunogen Sequence: MEPPASKVPEVPTAPATDAA PKRVEIQMPKPAEAPTAPSP AQTLENSEPAPVSQLQSRLE PKPQPPVAEATPRSQEATEA APSCVGDMADTPRDAGLKQA PASRNEKAPV
Tissue Specificity Widely expressed. Isoforms are differentially expressed in testes, kidney, liver heart, spleen, brain, peripheral blood leukocytes, skeletal muscle and kidney. Specific isoforms appear to demonstrate tissue specificity. Isoform 5 is the most highly expressed in fetal tissue. Isoform 1 is detected in all tissues except the brain and thymus, while isoform 2, isoform 3, and isoform 4 are detected at low levels in approximately half of the fetal tissues.
Function Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.
Protein Name Septin-9
Mll Septin-Like Fusion Protein Msf-A
Mll Septin-Like Fusion Protein
Ovarian/Breast Septin
Ov/Br Septin
Septin D1
Cellular Localisation Cytoplasm
Cytoskeleton
In An Epithelial Cell Line
Concentrates At Cell-Cell Contact Areas
After Tgf-Beta1 Treatment And Induction Of Epithelial To Mesenchymal Transition
Colocalizes Partly With Actin Stress Fibers
During Bacterial Infection
Displays A Collar Shape Structure Next To Actin At The Pole Of Invading Bacteria
Alternative Antibody Names Anti-Septin-9 antibody
Anti-Mll Septin-Like Fusion Protein Msf-A antibody
Anti-Mll Septin-Like Fusion Protein antibody
Anti-Ovarian/Breast Septin antibody
Anti-Ov/Br Septin antibody
Anti-Septin D1 antibody
Anti-SEPTIN9 antibody
Anti-KIAA0991 antibody
Anti-MSF antibody
Anti-SEPT9 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance