• Immunofluorescence analysis of U-2 OS cells using Septin 3 Rabbit polyclonal antibody (STJ118978) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using Septin 3 Rabbit polyclonal antibody (STJ118978) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Septin 3 Rabbit polyclonal antibody (STJ118978) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SEPT3 antibody (179-358) (STJ118978)

SKU:
STJ118978

$151.88 - $708.08
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SEPTIN3
Gene ID: 55964
Uniprot ID: SEPT3_HUMAN
Immunogen Region: 179-358
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 179-358 of human Septin 3 (NP_663786.2).
Immunogen Sequence: SPTGHSLRPLDLEFMKHLSK VVNIIPVIAKADTMTLEEKS EFKQRVRKELEVNGIEFYPQ KEFDEDLEDKTENDKIRQES MPFAVVGSDKEYQVNGKRVL GRKTPWGIIEVENLNHCEFA LLRDFVIRTHLQDLKEVTHN IHYETYRAKRLNDNGGLPPG EGLLGTVLPPVPATPCPTAE
Tissue Specificity Brain-specific.
Post Translational Modifications Phosphorylated by PKG on serine residues. Phosphorylated by PKG on Ser-91.
Function Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential).
Protein Name Neuronal-Specific Septin-3
Cellular Localisation Cytoplasm
Cytoskeleton
Synapse
Alternative Antibody Names Anti-Neuronal-Specific Septin-3 antibody
Anti-SEPTIN3 antibody
Anti-SEP3 antibody
Anti-SEPT3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance