Western blot analysis of extracts of various cell lines, using SEMG1 antibody (STJ27440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SEMG1 antibody (24-310) (STJ27440)

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STJ27440
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Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SEMG1
Gene ID: 6406
Uniprot ID: SEMG1_HUMAN
Immunogen Region: 24-310
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 24-310 of human SEMG1 (NP_002998.1).
Immunogen Sequence: QKGGSKGRLPSEFSQFPHGQ KGQHYSGQKGKQQTESKGSF SIQYTYHVDANDHDQSRKSQ QYDLNALHKTTKSQRHLGGS QQLLHNKQEGRDHDKSKGHF HRVVIHHKGGKAHRGTQNPS QDQGNSPSGKGISSQYSNTE ERLWVHGLSKEQTSVSGAQK GRKQGGSQSSYVLQTEELVA NKQQRETKNSHQNKGHYQNV VEVREEHSSKVQTSLCPAHQ DKLQHGSKDIFSTQDELLV
Tissue Specificity Seminal vesicle.
Post Translational Modifications Transglutaminase substrate. Rapidly cleaved after ejaculation by KLK3/PSA, resulting in liquefaction of the semen coagulum and the progressive release of motile spermatozoa.
Function Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA. Alpha-inhibin-92 and alpha-inhibin-31, derived from the proteolytic degradation of semenogelin, inhibit the secretion of pituitary follicle-stimulating hormone.
Protein Name Semenogelin-1
Cancer/Testis Antigen 103
Semenogelin I
Sgi Cleaved Into - Alpha-Inhibin-92 - Alpha-Inhibin-31 - Seminal Basic Protein
Database Links Reactome: R-HSA-6803157
Reactome: R-HSA-977225
Cellular Localisation Secreted
Alternative Antibody Names Anti-Semenogelin-1 antibody
Anti-Cancer/Testis Antigen 103 antibody
Anti-Semenogelin I antibody
Anti-Sgi Cleaved Into - Alpha-Inhibin-92 - Alpha-Inhibin-31 - Seminal Basic Protein antibody
Anti-SEMG1 antibody
Anti-SEMG antibody

Information sourced from Uniprot.org

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