• Immunofluorescence analysis of L929 cells using SEMA4A Rabbit polyclonal antibody (STJ11102878) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using SEMA4A Rabbit polyclonal antibody (STJ11102878) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from HT-29 cells, using SEMA4A Rabbit polyclonal antibody (STJ11102878) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SEMA4A antibody (320-580) (STJ11102878)

SKU:
STJ11102878

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SEMA4A (320-580) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SEMA4A
Gene ID: 64218
Uniprot ID: SEM4A_HUMAN
Immunogen Region: 320-580
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 320-580 of human SEMA4A (NP_071762.2).
Immunogen Sequence: IYAVFTSQWQVGGTRSSAVC AFSLLDIERVFKGKYKELNK ETSRWTTYRGPETNPRPGSC SVGPSSDKALTFMKDHFLMD EQVVGTPLLVKSGVEYTRLA VETAQGLDGHSHLVMYLGTT TGSLHKAVVSGDSSAHLVEE IQLFPDPEPVRNLQLAPTQG AVFVGFSGGVWRVPRANCSV YESCVDCVLARDPHCAWDPE SRTCCLLSAPNLNSWKQDME RGNPEWACASGPMSRSLRP
Function Cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 that plays an important role in cell-cell signaling. Regulates glutamatergic and GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner and promotes the development of excitatory synapses in a PLXNB2-dependent manner. Plays a role in priming antigen-specific T-cells, promotes differentiation of Th1 T-helper cells, and thereby contributes to adaptive immunity. Promotes phosphorylation of TIMD2. Inhibits angiogenesis. Promotes axon growth cone collapse. Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
Protein Name Semaphorin-4a
Semaphorin-B
Sema B
Database Links Reactome: R-HSA-416700
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Semaphorin-4a antibody
Anti-Semaphorin-B antibody
Anti-Sema B antibody
Anti-SEMA4A antibody
Anti-SEMAB antibody
Anti-SEMB antibody
Anti-UNQ783 antibody
Anti-PRO1317 antibody

Information sourced from Uniprot.org

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