• Immunofluorescence analysis of L929 cells using SEC23IP Rabbit polyclonal antibody (STJ117593) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using SEC23IP Rabbit polyclonal antibody (STJ117593) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using SEC23IP Rabbit polyclonal antibody (STJ117593) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SEC23IP in paraffin-embedded Human liver cancer using SEC23IP Rabbit polyclonal antibody (STJ117593) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SEC23IP Rabbit polyclonal antibody (STJ117593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-SEC23IP antibody (600-940) (STJ117593)

SKU:
STJ117593

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SEC23IP (600-940) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SEC23IP
Gene ID: 11196
Uniprot ID: S23IP_HUMAN
Immunogen Region: 600-940
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 600-940 of human SEC23IP (NP_009121.1).
Immunogen Sequence: NLSKCPGPLAVANGVVKQLH FQEKQMPEEPKLTLDESYDL VVENKEVLTLQETLEALSLS EYFSTFEKEKIDMESLLMCT VDDLKEMGIPLGPRKKIANF VEHKAAKLKKAASEKKAVAA TSTKGQEQSAQKTKDMASLP SESNEPKRKLPVGACVSSVC VNYESFEVGAGQVSVAYNSL DFEPEIFFALGSPIAMFLTI RGVDRIDENYSLPTCKGFFN IYHPLDPVAYRLEPMIVPD
Tissue Specificity Ubiquitously expressed with stronger levels detected in heart, liver and skeletal muscle.
Function Plays a role in the organization of endoplasmic reticulum exit sites. Specifically binds to phosphatidylinositol 3-phosphate (PI(3)P), phosphatidylinositol 4-phosphate (PI(4)P) and phosphatidylinositol 5-phosphate (PI(5)P).
Protein Name Sec23-Interacting Protein
P125
Database Links Reactome: R-HSA-204005
Cellular Localisation Cytoplasmic Vesicle
Copii-Coated Vesicle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Endoplasmic Reticulum
Alternative Antibody Names Anti-Sec23-Interacting Protein antibody
Anti-P125 antibody
Anti-SEC23IP antibody
Anti-MSTP053 antibody

Information sourced from Uniprot.org

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