• Immunofluorescence analysis of NIH/3T3 cells using SDHC Rabbit polyclonal antibody (STJ11104152) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using SDHC Rabbit polyclonal antibody (STJ11104152) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A-431 cells using SDHC Rabbit polyclonal antibody (STJ11104152) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using SDHC Rabbit polyclonal antibody (STJ11104152) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SDHC antibody (STJ11104152) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SDHC antibody (1-100) (STJ11104152)

SKU:
STJ11104152

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Succinate Dehydrogenase Cytochrome B560 Subunit-Mitochondrial (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SDHC
Gene ID: 6391
Uniprot ID: C560_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SDHC (NP_002992.1).
Immunogen Sequence: MAALLLRHVGRHCLRAHFSP QLCIRNAVPLGTTAKEEMER FWNKNIGSNRPLSPHITIYS WSLPMAMSICHRGTGIALSA GVSLFGMSALLLPGNFESYL
Function Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
Protein Name Succinate Dehydrogenase Cytochrome B560 Subunit - Mitochondrial
Integral Membrane Protein Cii-3
Qps-1
Qps1
Succinate Dehydrogenase Complex Subunit C
Succinate-Ubiquinone Oxidoreductase Cytochrome B Large Subunit
Cybl
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-71403
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Succinate Dehydrogenase Cytochrome B560 Subunit - Mitochondrial antibody
Anti-Integral Membrane Protein Cii-3 antibody
Anti-Qps-1 antibody
Anti-Qps1 antibody
Anti-Succinate Dehydrogenase Complex Subunit C antibody
Anti-Succinate-Ubiquinone Oxidoreductase Cytochrome B Large Subunit antibody
Anti-Cybl antibody
Anti-SDHC antibody
Anti-CYB560 antibody
Anti-SDH3 antibody

Information sourced from Uniprot.org

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