• Immunofluorescence analysis of NIH/3T3 cells using SDHC Rabbit polyclonal antibody (STJ11104152) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using SDHC Rabbit polyclonal antibody (STJ11104152) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A-431 cells using SDHC Rabbit polyclonal antibody (STJ11104152) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using SDHC Rabbit polyclonal antibody (STJ11104152) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SDHC antibody (STJ11104152) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SDHC antibody (1-100) (STJ11104152)

SKU:
STJ11104152

£112.50 - £524.50
Current Stock:
Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SDHC
Gene ID: 6391
Uniprot ID: C560_HUMAN
Immunogen Region: 1-100
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SDHC (NP_002992.1).
Immunogen Sequence: MAALLLRHVGRHCLRAHFSP QLCIRNAVPLGTTAKEEMER FWNKNIGSNRPLSPHITIYS WSLPMAMSICHRGTGIALSA GVSLFGMSALLLPGNFESYL
Function Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate. Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate.
Protein Name Succinate Dehydrogenase Cytochrome B560 Subunit - Mitochondrial
Integral Membrane Protein Cii-3
Malate Dehydrogenase Quinone Cytochrome B560 Subunit
Qps-1
Qps1
Succinate Dehydrogenase Complex Subunit C
Succinate-Ubiquinone Oxidoreductase Cytochrome B Large Subunit
Cybl
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-71403
Reactome: R-HSA-9854311
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Succinate Dehydrogenase Cytochrome B560 Subunit - Mitochondrial antibody
Anti-Integral Membrane Protein Cii-3 antibody
Anti-Malate Dehydrogenase Quinone Cytochrome B560 Subunit antibody
Anti-Qps-1 antibody
Anti-Qps1 antibody
Anti-Succinate Dehydrogenase Complex Subunit C antibody
Anti-Succinate-Ubiquinone Oxidoreductase Cytochrome B Large Subunit antibody
Anti-Cybl antibody
Anti-SDHC antibody
Anti-CYB560 antibody
Anti-SDH3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance