• STJ71003 staining of HFF cell lysate with B) 100nM siRNA or A) control siRNA. Detected by chemiluminescence. Data kindly provided by Laura Papp and Kum Kum Khanna, Brisbane, Australia.
  • STJ71003 (3µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Anti-SBP2 antibody (Internal) (STJ71003)

SKU:
STJ71003-100

£312.50
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Host: Goat
Applications: Pep-ELISA/WB/IP/IHC/ICC/IF
Reactivity: Human/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-SBP2 (Internal) is suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:64000.
WB: Approx 120kDa band detected in HFF cell lysates. Data kindly provided by Laura Papp and Kum Kum Khanna. The observed molecular weight corresponds to earlier findings in literature with
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SECISBP2
Gene ID: 79048
Uniprot ID: SEBP2_HUMAN
Immunogen Region: Internal
Accession Number: NP_076982.3
Immunogen Sequence: KERQERKQRLQEN
Function mRNA-binding protein that binds to the SECIS (selenocysteine insertion sequence) element present in the 3'-UTR of mRNAs encoding selenoproteins and facilitates the incorporation of the rare amino acid selenocysteine. Insertion of selenocysteine at UGA codons is mediated by SECISBP2 and EEFSEC: SECISBP2 (1) specifically binds the SECIS sequence once the 80S ribosome encounters an in-frame UGA codon and (2) contacts the RPS27A/eS31 of the 40S ribosome before ribosome stalling. (3) GTP-bound EEFSEC then delivers selenocysteinyl-tRNA(Sec) to the 80S ribosome and adopts a preaccommodated state conformation. (4) After GTP hydrolysis, EEFSEC dissociates from the assembly, selenocysteinyl-tRNA(Sec) accommodates, and peptide bond synthesis and selenoprotein elongation occur.
Protein Name Selenocysteine Insertion Sequence-Binding Protein 2
Secis-Binding Protein 2
Database Links Reactome: R-HSA-2408557
Cellular Localisation Isoform 1: Nucleus
Isoform 2: Mitochondrion
Alternative Antibody Names Anti-Selenocysteine Insertion Sequence-Binding Protein 2 antibody
Anti-Secis-Binding Protein 2 antibody
Anti-SECISBP2 antibody
Anti-SBP2 antibody

Information sourced from Uniprot.org

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