• Immunoprecipitation of SARS-CoV-2 NSP10 from 300 µg extracts of 293T cells transfected with SARS-CoV-2 NSP10 was performed using 2 µg of SARS-CoV-2 NSP10 Rabbit polyclonal antibody (STJ11103272). Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using SARS-CoV-2 NSP10 Rabbit polyclonal antibody (STJ11103272) at a dilution of 1:4000.
  • Western blot analysis of extracts of normal 293T cells, 293T transfected with NSP10 Protein and 293T transfected with SOST Protein, using SARS-CoV-2 NSP10 Rabbit polyclonal antibody (STJ11103272) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SARS-CoV-2-NSP10 antibody (1-139) (STJ11103272)

SKU:
STJ11103272

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Host: Rabbit
Applications: WB
Reactivity: SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SARS-CoV-2-NSP10 (1-139) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-139
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-139 of coronavirus NSP10 (YP_009725306.1).
Immunogen Sequence: AGNATEVPANSTVLSFCAFA VDAAKAYKDYLASGGQPITN CVKMLCTHTGTGQAITVTPE ANMDQESFGGASCCLYCRCH IDHPNPKGFCDLKGKYVQIP TTCANDPVGFTLKNTVCTVC GMWKGYGCSCDQLREPMLQ
Background This gene encodes a cysteine-rich protein that contains two LIM domains but lacks a DNA-binding homeodomain. The encoded protein may play a role as a transcriptional regulator or as an oncogene.

Information sourced from Uniprot.org

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