• Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using SARS Rabbit polyclonal antibody (STJ115313) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using SARS Rabbit polyclonal antibody (STJ115313) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using SARS Rabbit polyclonal antibody (STJ115313) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SARS antibody (STJ115313) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SARS antibody (1-300) (STJ115313)

SKU:
STJ115313

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SARS (1-300) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SARS1
Gene ID: 6301
Uniprot ID: SYSC_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SARS (NP_006504.2).
Immunogen Sequence: MVLDLDLFRVDKGGDPALIR ETQEKRFKDPGLVDQLVKAD SEWRRCRFRADNLNKLKNLC SKTIGEKMKKKEPVGDDESV PENVLSFDDLTADALANLKV SQIKKVRLLIDEAILKCDAE RIKLEAERFENLREIGNLLH PSVPISNDEDVDNKVERIWG DCTVRKKYSHVDLVVMVDGF EGEKGAVVAGSRGYFLKGVL VFLEQALIQYALRTLGSRGY IPIYTPFFMRKEVMQEVAQ
Tissue Specificity Brain.
Function Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction: serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser). Is probably also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). In the nucleus, binds to the VEGFA core promoter and prevents MYC binding and transcriptional activation by MYC. Recruits SIRT2 to the VEGFA promoter, promoting deacetylation of histone H4 at 'Lys-16' (H4K16). Thereby, inhibits the production of VEGFA and sprouting angiogenesis mediated by VEGFA.
Protein Name Serine--Trna Ligase - Cytoplasmic
Seryl-Trna Synthetase
Serrs
Seryl-Trna(Ser/Sec Synthetase
Database Links Reactome: R-HSA-2408557
Reactome: R-HSA-379716
Cellular Localisation Cytoplasm
Nucleus
Predominantly Cytoplasmic
But A Minor Proportion Is Also Found In The Nucleus
Alternative Antibody Names Anti-Serine--Trna Ligase - Cytoplasmic antibody
Anti-Seryl-Trna Synthetase antibody
Anti-Serrs antibody
Anti-Seryl-Trna(Ser/Sec Synthetase antibody
Anti-SARS1 antibody
Anti-SARS antibody
Anti-SERS antibody

Information sourced from Uniprot.org

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