• Immunofluorescence analysis of paraffin-embedded rat testis using Sall4 Rabbit polyclonal antibody (STJ118633) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Sall4 in paraffin-embedded Mouse testis using Sall4 Rabbit polyclonal antibody (STJ118633) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Sall4 in paraffin-embedded Rat testis using Sall4 Rabbit polyclonal antibody (STJ118633) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Sall4 in paraffin-embedded Human testis using Sall4 Rabbit polyclonal antibody (STJ118633) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Sall4 Rabbit polyclonal antibody (STJ118633) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SALL4 antibody (853-1053) (STJ118633)

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STJ118633

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SALL4 (853-1053) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SALL4
Gene ID: 57167
Uniprot ID: SALL4_HUMAN
Immunogen Region: 853-1053
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 853-1053 of human Sall4 (NP_065169.1).
Immunogen Sequence: PGMTPLLAAQPRRQAKQHGC TRCGKNFSSASALQIHERTH TGEKPFVCNICGRAFTTKGN LKVHYMTHGANNNSARRGRK LAIENTMALLGTDGKRVSEI FPKEILAPSVNVDPVVWNQY TSMLNGGLAVKTNEISVIQS GGVPTLPVSLGATSVVNNAT VSKMDGSQSGISADVEKPSA TDGVPKHQFPHFLEENKIAV S
Tissue Specificity Expressed in testis. Constitutively expressed in acute myeloid leukemia (AML).
Post Translational Modifications Isoform SALL4B exists primarily as a ubiquitinated form. Sumoylation with both SUMO1 and SUMO2 regulates the stability, subcellular localization, transcriptional activity, and may reduce interaction with POU5F1/OCT4.
Function Transcription factor with a key role in the maintenance and self-renewal of embryonic and hematopoietic stem cells.
Protein Name Sal-Like Protein 4
Zinc Finger Protein 797
Zinc Finger Protein Sall4
Database Links Reactome: R-HSA-2892247
Reactome: R-HSA-452723
Reactome: R-HSA-8943724
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Sal-Like Protein 4 antibody
Anti-Zinc Finger Protein 797 antibody
Anti-Zinc Finger Protein Sall4 antibody
Anti-SALL4 antibody
Anti-ZNF797 antibody

Information sourced from Uniprot.org

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