Host: | Rabbit |
Applications: | WB/IHC/IF |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit polyclonal antibody anti-SAFB (1-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
Purification: | Affinity purification |
Dilution Range: | WB 1:500-1:2000 IHC-P 1:50-1:200 IF/ICC 1:50-1:200 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | SAFB |
Gene ID: | 6294 |
Uniprot ID: | SAFB1_HUMAN |
Immunogen Region: | 1-200 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human SAFB (NP_001188267.1). |
Immunogen Sequence: | MAETLSGLGDSGAAGAAALS SASSETGTRRLSDLRVIDLR AELRKRNVDSSGNKSVLMER LKKAIEDEGGNPDEIEITSE GNKKTSKRSSKGRKPEEEGV EDNGLEENSGDGQEDVETSL ENLQDIDIMDISVLDEAEID NGSVADCVEDDDADNLQESL SDSRELVEGEMKELPEQLQE HAIEDKETINNLDTSSSDFT |
Tissue Specificity | Ubiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines. |
Post Translational Modifications | Sumoylated by PIAS1 with SUMO1 and SUMO2/3, desumoylated by SENP1. Sumoylation is required for transcriptional repressor activity. |
Function | Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing. Functions as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Thereby acts as a negative regulator of cell proliferation. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter. |
Protein Name | Scaffold Attachment Factor B1 Saf-B Saf-B1 Hsp27 Estrogen Response Element-Tata Box-Binding Protein Hsp27 Ere-Tata-Binding Protein |
Database Links | Reactome: R-HSA-3899300 |
Cellular Localisation | Nucleus |
Alternative Antibody Names | Anti-Scaffold Attachment Factor B1 antibody Anti-Saf-B antibody Anti-Saf-B1 antibody Anti-Hsp27 Estrogen Response Element-Tata Box-Binding Protein antibody Anti-Hsp27 Ere-Tata-Binding Protein antibody Anti-SAFB antibody Anti-HAP antibody Anti-HET antibody Anti-SAFB1 antibody |
Information sourced from Uniprot.org