• Western blot analysis of extracts of various cell lines, using SAFB antibody (STJ110236) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of U-2 OS cells using SAFB antibody (STJ110236) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human colon using SAFB rabbit polyclonal antibody (STJ110236) at dilution of 1:200 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human colon using SAFB Rabbit polyclonal antibody (STJ110236) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U-2 OS cells using SAFB antibody (STJ110236) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using SAFB antibody (STJ110236) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SAFB antibody (1-200) (STJ110236)

SKU:
STJ110236

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SAFB (1-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SAFB
Gene ID: 6294
Uniprot ID: SAFB1_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human SAFB (NP_001188267.1).
Immunogen Sequence: MAETLSGLGDSGAAGAAALS SASSETGTRRLSDLRVIDLR AELRKRNVDSSGNKSVLMER LKKAIEDEGGNPDEIEITSE GNKKTSKRSSKGRKPEEEGV EDNGLEENSGDGQEDVETSL ENLQDIDIMDISVLDEAEID NGSVADCVEDDDADNLQESL SDSRELVEGEMKELPEQLQE HAIEDKETINNLDTSSSDFT
Tissue Specificity Ubiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.
Post Translational Modifications Sumoylated by PIAS1 with SUMO1 and SUMO2/3, desumoylated by SENP1. Sumoylation is required for transcriptional repressor activity.
Function Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing. Functions as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Thereby acts as a negative regulator of cell proliferation. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter.
Protein Name Scaffold Attachment Factor B1
Saf-B
Saf-B1
Hsp27 Estrogen Response Element-Tata Box-Binding Protein
Hsp27 Ere-Tata-Binding Protein
Database Links Reactome: R-HSA-3899300
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Scaffold Attachment Factor B1 antibody
Anti-Saf-B antibody
Anti-Saf-B1 antibody
Anti-Hsp27 Estrogen Response Element-Tata Box-Binding Protein antibody
Anti-Hsp27 Ere-Tata-Binding Protein antibody
Anti-SAFB antibody
Anti-HAP antibody
Anti-HET antibody
Anti-SAFB1 antibody

Information sourced from Uniprot.org

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