• Chromatin immunoprecipitation analysis of extracts from HepG2 cells, using RXR Alpha antibody (STJ11101642) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunoprecipitation analysis of 300 Mu g extracts from HepG2 cells using 3 Mu g RXR Alpha antibody (STJ11101642). Western blot was performed from the immunoprecipitate using RXR Alpha antibody (STJ11101642) at a dilution of 1:1000.
  • Confocal imaging of HeLa cells using RXR Alpha Rabbit monoclonal antibody (STJ11101642, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Western blot analysis of various lysates using RXR Alpha Rabbit monoclonal antibody (STJ11101642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using RXR Alpha Rabbit monoclonal antibody (STJ11101642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Chromatin immunoprecipitation was performed with 25 Mu g of cross-linked chromatin from HepG2 cells using 5 Mu g of RXR Alpha Rabbit monoclonal antibody (STJ11101642). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of RXR Alpha in the representative genomic region surrounding ECH1 gene.
  • Chromatin immunoprecipitation was performed with 25 Mu g of cross-linked chromatin from HepG2 cells using 5 Mu g of RXR Alpha Rabbit monoclonal antibody (STJ11101642). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of RXR Alpha across chromosome 19 (upper panel) and the genomic region encompassing ECH1, a representative gene enriched in RXR Alpha (lower panel).

Anti-RXRA antibody (1-100) [S2MR] (STJ11101642)

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STJ11101642

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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-RXR Alpha (1-100) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:500-1:1000
ChIP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RXRA
Gene ID: 6256
Uniprot ID: RXRA_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RXR Alpha (P19793).
Immunogen Sequence: MDTKHFLPLDFSTQVNSSLT SPTGRGSMAAPSLHPSLGPG IGSPGQLHSPISTLSSPING MGPPFSVISSPMGPHSMSVP TTPTLGFSTGSPQLSSPMNP
Tissue Specificity Expressed in lung fibroblasts (at protein level). Expressed in monocytes. Highly expressed in liver, also found in kidney and brain.
Post Translational Modifications Acetylated by EP300.acetylation enhances DNA binding and transcriptional activity. Phosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constitutively phosphorylated on Ser-21 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260. Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA. Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6.
Function Receptor for retinoic acid that acts as a transcription factor. Forms homo- or heterodimers with retinoic acid receptors (RARs) and binds to target response elements in response to their ligands, all-trans or 9-cis retinoic acid, to regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5 to regulate transcription. The high affinity ligand for retinoid X receptors (RXRs) is 9-cis retinoic acid. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone deacetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and coactivators are recruited leading to transcriptional activation. Serves as a common heterodimeric partner for a number of nuclear receptors, such as RARA, RARB and PPARA. The RXRA/RARB heterodimer can act as a transcriptional repressor or transcriptional activator, depending on the RARE DNA element context. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. Together with RARA, positively regulates microRNA-10a expression, thereby inhibiting the GATA6/VCAM1 signaling response to pulsatile shear stress in vascular endothelial cells. Acts as an enhancer of RARA binding to RARE DNA element. May facilitate the nuclear import of heterodimerization partners such as VDR and NR4A1. Promotes myelin debris phagocytosis and remyelination by macrophages. Plays a role in the attenuation of the innate immune system in response to viral infections, possibly by negatively regulating the transcription of antiviral genes such as type I IFN genes. Involved in the regulation of calcium signaling by repressing ITPR2 gene expression, thereby controlling cellular senescence.
Protein Name Retinoic Acid Receptor Rxr-Alpha
Nuclear Receptor Subfamily 2 Group B Member 1
Retinoid X Receptor Alpha
Database Links Reactome: R-HSA-1368082
Reactome: R-HSA-1368108
Reactome: R-HSA-159418
Reactome: R-HSA-192105
Reactome: R-HSA-193368
Reactome: R-HSA-193807
Reactome: R-HSA-1989781
Reactome: R-HSA-200425
Reactome: R-HSA-204174
Reactome: R-HSA-211976
Reactome: R-HSA-2151201
Reactome: R-HSA-2426168
Reactome: R-HSA-381340
Reactome: R-HSA-383280
Reactome: R-HSA-400206
Reactome: R-HSA-400253
Reactome: R-HSA-4090294
Reactome: R-HSA-5362517
Reactome: R-HSA-5617472
Reactome: R-HSA-9029558
Reactome: R-HSA-9029569
Reactome: R-HSA-9031525
Reactome: R-HSA-9031528
Reactome: R-HSA-9616222
Reactome: R-HSA-9623433
Reactome: R-HSA-9632974
Reactome: R-HSA-9707564
Reactome: R-HSA-9707616
Cellular Localisation Nucleus
Cytoplasm
Mitochondrion
Localization To The Nucleus Is Enhanced By Vitamin D3
Nuclear Localization May Be Enhanced By The Interaction With Heterodimerization Partner Vdr
Translocation To The Mitochondrion Upon Interaction With Nr4a1
Increased Nuclear Localization Upon Pulsatile Shear Stress
Alternative Antibody Names Anti-Retinoic Acid Receptor Rxr-Alpha antibody
Anti-Nuclear Receptor Subfamily 2 Group B Member 1 antibody
Anti-Retinoid X Receptor Alpha antibody
Anti-RXRA antibody
Anti-NR2B1 antibody

Information sourced from Uniprot.org

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