| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RWD domain-containing protein 3 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RWDD3 |
| Gene ID: |
25950 |
| Uniprot ID: |
RWDD3_HUMAN |
| Specificity: |
This antibody detects endogenous levels of RWDD3 at Human, Rat |
| Immunogen: |
Synthesized peptide derived from human RWDD3 |
| Function | Enhancer of SUMO conjugation. Via its interaction with UBE2I/UBC9, increases SUMO conjugation to proteins by promoting the binding of E1 and E2 enzymes, thioester linkage between SUMO and UBE2I/UBC9 and transfer of SUMO to specific target proteins which include HIF1A, PIAS, NFKBIA, NR3C1 and TOP1. Isoform 1 and isoform 2 positively regulate the NF-kappa-B signaling pathway by enhancing the sumoylation of NF-kappa-B inhibitor alpha (NFKBIA), promoting its stabilization which consequently leads to an increased inhibition of NF-kappa-B transcriptional activity. Isoform 1 and isoform 2 negatively regulate the hypoxia-inducible factor-1 alpha (HIF1A) signaling pathway by increasing the sumoylation of HIF1A, promoting its stabilization, transcriptional activity and the expression of its target gene VEGFA during hypoxia. Isoform 2 promotes the sumoylation and transcriptional activity of the glucocorticoid receptor NR3C1 and enhances the interaction of SUMO1 and NR3C1 with UBE2I/UBC9. Has no effect on ubiquitination. |
| Protein Name | Rwd Domain-Containing Protein 3Rwd Domain-Containing Sumoylation EnhancerRsume |
| Database Links | Reactome: R-HSA-3065678 |
| Cellular Localisation | NucleusCytoplasmColocalizes With Ubc9/Ube2i In Nuclear Spots |
| Alternative Antibody Names | Anti-Rwd Domain-Containing Protein 3 antibodyAnti-Rwd Domain-Containing Sumoylation Enhancer antibodyAnti-Rsume antibodyAnti-RWDD3 antibodyAnti-RSUME antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
