Anti-RWDD3 antibody (STJA0008469)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human RWDD3
General Information
| Short Description | Rabbit polyclonal anti-RWD domain-containing protein 3 for use in WB in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RWDD3 |
| Gene ID | 25950 |
| Uniprot ID | RWDD3_HUMAN |
| Immunogen | Synthesized peptide derived from human RWDD3 |
| Specificity | This antibody detects endogenous levels of RWDD3 at Human, Rat |
Additional Info
| Function | Enhancer of SUMO conjugation. Via its interaction with UBE2I/UBC9, increases SUMO conjugation to proteins by promoting the binding of E1 and E2 enzymes, thioester linkage between SUMO and UBE2I/UBC9 and transfer of SUMO to specific target proteins which include HIF1A, PIAS, NFKBIA, NR3C1 and TOP1. Isoform 1 and isoform 2 positively regulate the NF-kappa-B signaling pathway by enhancing the sumoylation of NF-kappa-B inhibitor alpha (NFKBIA), promoting its stabilization which consequently leads to an increased inhibition of NF-kappa-B transcriptional activity. Isoform 1 and isoform 2 negatively regulate the hypoxia-inducible factor-1 alpha (HIF1A) signaling pathway by increasing the sumoylation of HIF1A, promoting its stabilization, transcriptional activity and the expression of its target gene VEGFA during hypoxia. Isoform 2 promotes the sumoylation and transcriptional activity of the glucocorticoid receptor NR3C1 and enhances the interaction of SUMO1 and NR3C1 with UBE2I/UBC9. Has no effect on ubiquitination. |
| Protein Name | Rwd Domain-Containing Protein 3Rwd Domain-Containing Sumoylation EnhancerRsume |
| Database Links | Reactome: R-HSA-3065678 |
| Cellular Localisation | NucleusCytoplasmColocalizes With Ubc9/Ube2i In Nuclear Spots |
| Alternative Antibody Names | Anti-Rwd Domain-Containing Protein 3 antibodyAnti-Rwd Domain-Containing Sumoylation Enhancer antibodyAnti-Rsume antibodyAnti-RWDD3 antibodyAnti-RSUME antibody |
Information sourced from Uniprot.org