• Western blot analysis of extracts of various cell lines, using RRAGD antibody (STJ112020) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using RRAGD antibody (STJ112020) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver cancer using RRAGD antibody (STJ112020) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using RRAGD antibody (STJ112020) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-RRAGD antibody (181-400) (STJ112020)

SKU:
STJ112020

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RRAGD (181-400) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:4000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RRAGD
Gene ID: 58528
Uniprot ID: RRAGD_HUMAN
Immunogen Region: 181-400
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 181-400 of human RRAGD (NP_067067.1).
Immunogen Sequence: VDGLSDDHKIETQRDIHQRA NDDLADAGLEKIHLSFYLTS IYDHSIFEAFSKVVQKLIPQ LPTLENLLNIFISNSGIEKA FLFDVVSKIYIATDSTPVDM QTYELCCDMIDVVIDISCIY GLKEDGAGTPYDKESTAIIK LNNTTVLYLKEVTKFLALVC FVREESFERKGLIDYNFHCF RKAIHEVFEVRMKVVKSRKV QNRLQKKKRATPNGTPRVLL
Function Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RagA/RRAGA or RagB/RRAGB and cycles between an inactive GTP-bound and an active GDP-bound form: RagD/RRAGD is in its active form when GDP-bound RagD/RRAGD forms a complex with GTP-bound RagA/RRAGA (or RagB/RRAGB) and in an inactive form when GTP-bound RagD/RRAGD heterodimerizes with GDP-bound RagA/RRAGA (or RagB/RRAGB). In its active form, promotes the recruitment of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the MTOR signaling cascade by amino acids. Also plays a central role in the non-canonical mTORC1 complex, which acts independently of RHEB and specifically mediates phosphorylation of MiT/TFE factors TFEB and TFE3: GDP-bound RagD/RRAGD mediates recruitment of MiT/TFE factors TFEB and TFE3.
Protein Name Ras-Related Gtp-Binding Protein D
Rag D
Ragd
Database Links Reactome: R-HSA-1632852
Reactome: R-HSA-165159
Reactome: R-HSA-166208
Reactome: R-HSA-380972
Reactome: R-HSA-5628897
Reactome: R-HSA-8943724
Reactome: R-HSA-9639288
Cellular Localisation Cytoplasm
Nucleus
Lysosome Membrane
Predominantly Cytoplasmic
Recruited To The Lysosome Surface By The Ragulator Complex
May Shuttle Between The Cytoplasm And Nucleus
Depending On The Bound Nucleotide State Of Associated Rraga
Alternative Antibody Names Anti-Ras-Related Gtp-Binding Protein D antibody
Anti-Rag D antibody
Anti-Ragd antibody
Anti-RRAGD antibody

Information sourced from Uniprot.org

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