| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RPS6KA4 (673-772) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:20-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RPS6KA4 |
| Gene ID: |
8986 |
| Uniprot ID: |
KS6A4_HUMAN |
| Immunogen Region: |
673-772 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 673-772 of human RPS6KA4 (NP_003933.1). |
| Immunogen Sequence: |
WLQDGSARSSPPLRTPDVLE SSGPAVRSGLNATFMAFNRG KREGFFLKSVENAPLAKRRK QKLRSATASRRGSPAPANPG RAPVASKGAPRRANGPLPPS |
| Post Translational Modifications | Ser-343 and Thr-568 phosphorylation is required for kinase activity. Ser-343 and Ser-196 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. Phosphorylated at Ser-343, Thr-568 and Thr-687 by MAPK1/ERK2, MAPK3/ERK1 and MAPK14/p38-alpha. Autophosphorylated at Ser-737 and Ser-745 by the N-terminal kinase domain. |
| Function | Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors. |
| Protein Name | Ribosomal Protein S6 Kinase Alpha-4S6k-Alpha-490 Kda Ribosomal Protein S6 Kinase 4Nuclear Mitogen- And Stress-Activated Protein Kinase 2Ribosomal Protein Kinase BRskb |
| Database Links | Reactome: R-HSA-437239 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Ribosomal Protein S6 Kinase Alpha-4 antibodyAnti-S6k-Alpha-4 antibodyAnti-90 Kda Ribosomal Protein S6 Kinase 4 antibodyAnti-Nuclear Mitogen- And Stress-Activated Protein Kinase 2 antibodyAnti-Ribosomal Protein Kinase B antibodyAnti-Rskb antibodyAnti-RPS6KA4 antibodyAnti-MSK2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
