• Immunofluorescence analysis of MCF7 cells using RPLP1 Rabbit polyclonal antibody (STJ28808). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using RPLP1 Rabbit polyclonal antibody (STJ28808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-RPLP1 antibody (1-114) (STJ28808)

SKU:
STJ28808

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat/Zebrafish
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPLP1 (1-114) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPLP1
Gene ID: 6176
Uniprot ID: RLA1_HUMAN
Immunogen Region: 1-114
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human RPLP1 (NP_000994.1).
Immunogen Sequence: MASVSELACIYSALILHDDE VTVTEDKINALIKAAGVNVE PFWPGLFAKALANVNIGSLI CNVGAGGPAPAAGAAPAGGP APSTAAAPAEEKKVEAKKEE SEESDDDMGFGLFD
Post Translational Modifications Ubiquitinated at Lys-92 and Lys-93 by RNF14 and RNF25 in response to ribosome collisions (ribosome stalling).
Function Plays an important role in the elongation step of protein synthesis.
Protein Name Large Ribosomal Subunit Protein P1
60s Acidic Ribosomal Protein P1
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6791226
Reactome: R-HSA-72689
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein P1 antibody
Anti-60s Acidic Ribosomal Protein P1 antibody
Anti-RPLP1 antibody
Anti-RRP1 antibody

Information sourced from Uniprot.org

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