• Confocal immunofluorescence analysis of A-431 cells using RPL21 Rabbit polyclonal antibody (STJ116467) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Confocal immunofluorescence analysis of HeLa cells using RPL21 Rabbit polyclonal antibody (STJ116467) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of RPL21 in paraffin-embedded rat brain using RPL21 Rabbit polyclonal antibody (STJ116467) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using RPL21 Rabbit polyclonal antibody (STJ116467) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-RPL21 antibody (1-160) (STJ116467)

SKU:
STJ116467

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPL21 (1-160) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPL21
Gene ID: 6144
Uniprot ID: RL21_HUMAN
Immunogen Region: 1-160
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human RPL21 (NP_000973.2).
Immunogen Sequence: MTNTKGKRRGTRYMFSRPFR KHGVVPLATYMRIYKKGDIV DIKGMGTVQKGMPHKCYHGK TGRVYNVTQHAVGIVVNKQV KGKILAKRINVRIEHIKHSK SRDSFLKRVKENDQKKKEAK EKGTWVQLKRQPAPPREAHF VRTNGKEPELLEPIPYEFMA
Function Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Protein Name Large Ribosomal Subunit Protein El21
60s Ribosomal Protein L21
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6791226
Reactome: R-HSA-72689
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Cytosol
Endoplasmic Reticulum
Detected On Cytosolic Polysomes
Detected In Ribosomes That Are Associated With The Rough Endoplasmic Reticulum
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein El21 antibody
Anti-60s Ribosomal Protein L21 antibody
Anti-RPL21 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance