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Anti-RPB3/POLR2C antibody (1-100) [S8MR] (STJ11103678)

Western blot analysis of extracts of various cell lines, using RPB3/POLR2C antibody (STJ11103678) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using RPB3/POLR2C Rabbit monoclonal antibody (STJ11103678) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry of paraffin-embedded rat lung using RPB3/POLR2C rabbit monoclonal antibody (STJ11103678) at dilution of 1:100 (40x lens).
Immunohistochemistry analysis of paraffin-embedded rat lung using RPB3/POLR2C Rabbit monoclonal antibody (STJ11103678) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry of paraffin-embedded human lung cancer using RPB3/POLR2C rabbit monoclonal antibody (STJ11103678) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using RPB3/POLR2C antibody (STJ11103678) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-RPB3/POLR2C antibody (1-100) [S8MR] (STJ11103678)

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STJ11103678

£129.50 - £574.50

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC-P/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Clonality:	Monoclonal
Clone ID:	S8MR
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification:	Affinity purification
Concentration:	Lot specific
Dilution Range:	WB:1:100-1:500 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	POLR2C
Gene ID:	5432
Uniprot ID:	RPB3_HUMAN
Immunogen Region:	1-100
Specificity:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPB3/POLR2C (P19387).
Immunogen Sequence:	MPYANQPTVRITELTDENVK FIIENTDLAVANSIRRVFIA EVPIIAIDWVQIDANSSVLH DEFIAHRLGLIPLISDDIVD KLQYSRDCTCEEFCPECSVE

Function	Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates.
Protein Name	Dna-Directed Rna Polymerase Ii Subunit Rpb3 Rna Polymerase Ii Subunit 3 Rna Polymerase Ii Subunit B3 Dna-Directed Rna Polymerase Ii 33 Kda Polypeptide Rpb33 Dna-Directed Rna Polymerase Ii Subunit C Rpb31
Database Links	Reactome: R-HSA-112382 Reactome: R-HSA-113418 Reactome: R-HSA-167152 Reactome: R-HSA-167158 Reactome: R-HSA-167160 Reactome: R-HSA-167161 Reactome: R-HSA-167162 Reactome: R-HSA-167172 Reactome: R-HSA-167200 Reactome: R-HSA-167238 Reactome: R-HSA-167242 Reactome: R-HSA-167243 Reactome: R-HSA-167246 Reactome: R-HSA-167287 Reactome: R-HSA-167290 Reactome: R-HSA-168325 Reactome: R-HSA-203927 Reactome: R-HSA-5578749 Reactome: R-HSA-5601884 Reactome: R-HSA-5617472 Reactome: R-HSA-674695 Reactome: R-HSA-6781823 Reactome: R-HSA-6781827 Reactome: R-HSA-6782135 Reactome: R-HSA-6782210 Reactome: R-HSA-6796648 Reactome: R-HSA-6803529 Reactome: R-HSA-6807505 Reactome: R-HSA-72086 Reactome: R-HSA-72163 Reactome: R-HSA-72165 Reactome: R-HSA-72203 Reactome: R-HSA-73776 Reactome: R-HSA-73779 Reactome: R-HSA-75953 Reactome: R-HSA-75955 Reactome: R-HSA-76042 Reactome: R-HSA-77075 Reactome: R-HSA-8851708 Reactome: R-HSA-9018519 Reactome: R-HSA-9670095
Cellular Localisation	Nucleus
Alternative Antibody Names	Anti-Dna-Directed Rna Polymerase Ii Subunit Rpb3 antibody Anti-Rna Polymerase Ii Subunit 3 antibody Anti-Rna Polymerase Ii Subunit B3 antibody Anti-Dna-Directed Rna Polymerase Ii 33 Kda Polypeptide antibody Anti-Rpb33 antibody Anti-Dna-Directed Rna Polymerase Ii Subunit C antibody Anti-Rpb31 antibody Anti-POLR2C antibody Anti-A-152E5.7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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