Western blot analysis of various lysates using RPB3/POLR2C Rabbit mAb (STJ11103678) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-RPB3/POLR2C antibody (1-100) [S8MR] (STJ11103678)

Name: Anti-RPB3/POLR2C antibody (1-100) [S8MR] (STJ11103678)
SKU: STJ11103678
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

ClonalityMonoclonal

HostRabbit

ConjugationUnconjugated

IsotypeIgG

STJ11103678

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General Information

Short Description	Rabbit monoclonal RPB3/POLR2C (1-100) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
Applications	WB/IHC-P/ELISA
Host	Rabbit
Reactivity	Human/Mouse/Rat
Note	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Clonality	Monoclonal
Clone ID	S8MR
Isotype	IgG
Conjugation	Unconjugated
Concentration	Lot specific
Purification	Affinity purification
Dilution Range	WB:1:100-1:500 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Formulation	PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage Instruction	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene Symbol	POLR2C
Gene ID	5432
Uniprot ID	RPB3_HUMAN
Immunogen Region	1-100
Immunogen Sequence	MPYANQPTVRITELTDENVK FIIENTDLAVANSIRRVFIA EVPIIAIDWVQIDANSSVLH DEFIAHRLGLIPLISDDIVD KLQYSRDCTCEEFCPECSVE
Specificity	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPB3/POLR2C (P19387).

Additional Info

Function	Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates.
Protein Name	Dna-Directed Rna Polymerase Ii Subunit Rpb3 Rna Polymerase Ii Subunit 3 Rna Polymerase Ii Subunit B3 Dna-Directed Rna Polymerase Ii 33 Kda Polypeptide Rpb33 Dna-Directed Rna Polymerase Ii Subunit C Rpb31
Database Links	Reactome: R-HSA-112382 Reactome: R-HSA-113418 Reactome: R-HSA-167152 Reactome: R-HSA-167158 Reactome: R-HSA-167160 Reactome: R-HSA-167161 Reactome: R-HSA-167162 Reactome: R-HSA-167172 Reactome: R-HSA-167200 Reactome: R-HSA-167238 Reactome: R-HSA-167242 Reactome: R-HSA-167243 Reactome: R-HSA-167246 Reactome: R-HSA-167287 Reactome: R-HSA-167290 Reactome: R-HSA-168325 Reactome: R-HSA-203927 Reactome: R-HSA-5578749 Reactome: R-HSA-5601884 Reactome: R-HSA-5617472 Reactome: R-HSA-674695 Reactome: R-HSA-6781823 Reactome: R-HSA-6781827 Reactome: R-HSA-6782135 Reactome: R-HSA-6782210 Reactome: R-HSA-6796648 Reactome: R-HSA-6803529 Reactome: R-HSA-6807505 Reactome: R-HSA-72086 Reactome: R-HSA-72163 Reactome: R-HSA-72165 Reactome: R-HSA-72203 Reactome: R-HSA-73776 Reactome: R-HSA-73779 Reactome: R-HSA-75953 Reactome: R-HSA-75955 Reactome: R-HSA-76042 Reactome: R-HSA-77075 Reactome: R-HSA-8851708 Reactome: R-HSA-9018519 Reactome: R-HSA-9670095
Cellular Localisation	Nucleus
Alternative Antibody Names	Anti-Dna-Directed Rna Polymerase Ii Subunit Rpb3 antibody Anti-Rna Polymerase Ii Subunit 3 antibody Anti-Rna Polymerase Ii Subunit B3 antibody Anti-Dna-Directed Rna Polymerase Ii 33 Kda Polypeptide antibody Anti-Rpb33 antibody Anti-Dna-Directed Rna Polymerase Ii Subunit C antibody Anti-Rpb31 antibody Anti-POLR2C antibody Anti-A-152E5.7 antibody

Information sourced from Uniprot.org

Citations

View product citations for antibody STJ11103678 on CiteAb