| Host: |
Rabbit |
| Applications: |
WB/ICC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Robo1 (C-Term) is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:500IHCICC 1:200IP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ROBO1 |
| Gene ID: |
6091 |
| Uniprot ID: |
ROBO1_HUMAN |
| Immunogen Region: |
C-Term |
| Specificity: |
The antibody detects a 180 kDa* band corresponding to Robo1 in Western blots of human HepG2 and HUVEC cells, as well as mouse C2C12 cells. |
| Immunogen: |
Robo1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human Robo1. This sequence has significant homology to the same region in rat and mouse Robo1, and has low homology to other Robo family membe |
| Post Translational Modifications | Ubiquitinated. May be deubiquitinated by USP33. |
| Function | Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. Plays a role in the regulation of cell migration via its interaction with MYO9B.inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development. |
| Protein Name | Roundabout Homolog 1Deleted In U Twenty TwentyH-Robo-1 |
| Database Links | Reactome: R-HSA-373752Reactome: R-HSA-376176Reactome: R-HSA-428540Reactome: R-HSA-428542Reactome: R-HSA-428543Reactome: R-HSA-428890Reactome: R-HSA-8985586Reactome: R-HSA-8985801Reactome: R-HSA-9010553 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionAxonEndoplasmic Reticulum-Golgi Intermediate Compartment MembraneSingle-Pass Membrane ProteinDetected At Growth Cones In Thalamus NeuronsPrrg4 Prevents Cell Surface Location And Both Colocalize In The Endoplasmic Reticulum/Golgi Adjacent To The Cell Nucleus |
| Alternative Antibody Names | Anti-Roundabout Homolog 1 antibodyAnti-Deleted In U Twenty Twenty antibodyAnti-H-Robo-1 antibodyAnti-ROBO1 antibodyAnti-DUTT1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
