Western blot analysis of various lysates using RNPEP Rabbit pAb (STJ25376) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-RNPEP antibody (371-650) (STJ25376)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ25376
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General Information

Short DescriptionRabbit polyclonal RNPEP (371-650) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:1000-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolRNPEP
Gene ID6051
Uniprot IDAMPB_HUMAN
Immunogen Region371-650
Immunogen SequenceEAATGRALLRQHMDITGEEN PLNKLRVKIEPGVDPDDTYN ETPYEKGFCFVSYLAHLVGD QDQFDSFLKAYVHEFKFRSI LADDFLDFYLEYFPELKKKR VDIIPGFEFDRWLNTPGWPP YLPDLSPGDSLMKPAEELAQ LWAAEELDMKAIEAVAISPW KTYQLVYFLDKILQKSPLPP GNVKKLGDTYPSISNARNAE LRLRWGQIVLKNDHQEDFWK VKEFLHNQGKQKYTLPLYH
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 371-650 of human RNPEP (NP_064601.3).

Additional Info

Function Exopeptidase which selectively removes arginine and/or lysine residues from the N-terminus of several peptide substrates including Arg(0)-Leu-enkephalin, Arg(0)-Met-enkephalin and Arg(-1)-Lys(0)-somatostatin-14. Can hydrolyze leukotriene A4 (LTA-4) into leukotriene B4 (LTB-4).
Protein Name Aminopeptidase B
Ap-B
Arginine Aminopeptidase
Arginyl Aminopeptidase
Database Links
Cellular Localisation Secreted
Alternative Antibody Names Anti-Aminopeptidase B antibody
Anti-Ap-B antibody
Anti-Arginine Aminopeptidase antibody
Anti-Arginyl Aminopeptidase antibody
Anti-RNPEP antibody
Anti-APB antibody

Information sourced from Uniprot.org

Citations

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