• Immunofluorescence analysis of U-2 OS cells using RNH1 Rabbit polyclonal antibody (STJ25374) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using RNH1 Rabbit polyclonal antibody (STJ25374) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using RNH1 Rabbit polyclonal antibody (STJ25374) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using RNH1 antibody (STJ25374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-RNH1 antibody (202-461) (STJ25374)

SKU:
STJ25374

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RNH1 (202-461) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNH1
Gene ID: 6050
Uniprot ID: RINI_HUMAN
Immunogen Region: 202-461
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 202-461 of human RNH1 (NP_002930.2).
Immunogen Sequence: EALKLESCGVTSDNCRDLCG IVASKASLRELALGSNKLGD VGMAELCPGLLHPSSRLRTL WIWECGITAKGCGDLCRVLR AKESLKELSLAGNELGDEGA RLLCETLLEPGCQLESLWVK SCSFTAACCSHFSSVLAQNR FLLELQISNNRLEDAGVREL CQGLGQPGSVLRVLWLADCD VSDSSCSSLAATLLANHSLR ELDLSNNCLGDAGILQLVES VRQPGCLLEQLVLYDIYWS
Post Translational Modifications The N-terminus is blocked. At least 30 of the 32 cysteine residues are in the reduced form.
Function Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play a role in redox homeostasis.
Protein Name Ribonuclease Inhibitor
Placental Ribonuclease Inhibitor
Placental Rnase Inhibitor
Ribonuclease/Angiogenin Inhibitor 1
Rai
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Ribonuclease Inhibitor antibody
Anti-Placental Ribonuclease Inhibitor antibody
Anti-Placental Rnase Inhibitor antibody
Anti-Ribonuclease/Angiogenin Inhibitor 1 antibody
Anti-Rai antibody
Anti-RNH1 antibody
Anti-PRI antibody
Anti-RNH antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance