Host: | Rabbit |
Applications: | WB/IHC-P/ELISA |
Reactivity: | Human/Mouse |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Clonality: | Monoclonal |
Clone ID: | S0MR |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
Purification: | Affinity purification |
Concentration: | Lot specific |
Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | RNF40 |
Gene ID: | 9810 |
Uniprot ID: | BRE1B_HUMAN |
Immunogen Region: | 231-431 |
Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 231-431 of human RNF40 (O75150). |
Immunogen Sequence: | RELGRENRRLQDLATQLQEK HHRISLEYSELQDKVTSAET KVLEMETTVEDLQWDIEKLR KREQKLNKHLAEALEQLNSG YYVSGSSSGFQGGQITLSMQ KFEMLNAELEENQELANSRM AELEKLQAELQGAVRTNERL KVALRSLPEEVVRETGEYRM LQAQFSLLYNESLQVKTQLD EARGLLLATKNSHLRHIEHM E |
Tissue Specificity | Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine. |
Function | Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B.reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. (Microbial infection) Promotes the human herpesvirus 8 (KSHV) lytic cycle by inducing the expression of lytic viral genes including the latency switch gene RTA/ORF50. |
Protein Name | E3 Ubiquitin-Protein Ligase Bre1b Bre1-B 95 Kda Retinoblastoma-Associated Protein Rbp95 Ring Finger Protein 40 Ring-Type E3 Ubiquitin Transferase Bre1b |
Database Links | Reactome: R-HSA-8866654 |
Cellular Localisation | Nucleus |
Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Bre1b antibody Anti-Bre1-B antibody Anti-95 Kda Retinoblastoma-Associated Protein antibody Anti-Rbp95 antibody Anti-Ring Finger Protein 40 antibody Anti-Ring-Type E3 Ubiquitin Transferase Bre1b antibody Anti-RNF40 antibody Anti-BRE1B antibody Anti-KIAA0661 antibody |
Information sourced from Uniprot.org