| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RNF220 |
| Gene ID: | 55182 |
| Uniprot ID: | RN220_HUMAN |
| Immunogen Region: | 267-566 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 267-566 of human RNF220 (NP_060620.2). |
| Immunogen Sequence: | PKSLLLSASIKREGESPTAS PHSSATDDLHHSDRYQTFLR VRANRQTRLNARIGKMKRRK QDEGQREGSCMAEDDAVDIE HENNNRFEEYEWCGQKRIRA TTLLEGGFRGSGFIMCSGKE NPDSDADLDVDGDDTLEYGK PQYTEADVIPCTGEEPGEAK EREALRGAVLNGGPPSTRIT PEFSKWASDEMPSTSNGESS KQEAMQKTCKNSDIEKITED SAVTTFEALKARVRELERQ |
| Tissue Specificity | Ubiquitously expressed. Abundant in brain and spinal cord, particularly in the cerebellum and cerebral cortex. In fetal tissues expressed in the cerebellum, spinal cord and cortex. |
| Post Translational Modifications | Auto-ubiquitinated.leads to proteasomal degradation. |
| Function | E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of SIN3B. Independently of its E3 ligase activity, acts as a CTNNB1 stabilizer through USP7-mediated deubiquitination of CTNNB1 promoting Wnt signaling. Plays a critical role in the regulation of nuclear lamina. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf220Ring Finger Protein 220Ring-Type E3 Ubiquitin Transferase Rnf220 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf220 antibodyAnti-Ring Finger Protein 220 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Rnf220 antibodyAnti-RNF220 antibodyAnti-C1orf164 antibody |
Information sourced from Uniprot.org