• Western blot analysis of various lysates, using RNF220 Rabbit polyclonal antibody (STJ118298) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-RNF220 antibody (267-566) (STJ118298)

SKU:
STJ118298

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RNF220 (267-566) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNF220
Gene ID: 55182
Uniprot ID: RN220_HUMAN
Immunogen Region: 267-566
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 267-566 of human RNF220 (NP_060620.2).
Immunogen Sequence: PKSLLLSASIKREGESPTAS PHSSATDDLHHSDRYQTFLR VRANRQTRLNARIGKMKRRK QDEGQREGSCMAEDDAVDIE HENNNRFEEYEWCGQKRIRA TTLLEGGFRGSGFIMCSGKE NPDSDADLDVDGDDTLEYGK PQYTEADVIPCTGEEPGEAK EREALRGAVLNGGPPSTRIT PEFSKWASDEMPSTSNGESS KQEAMQKTCKNSDIEKITED SAVTTFEALKARVRELERQ
Tissue Specificity Ubiquitously expressed. Abundant in brain and spinal cord, particularly in the cerebellum and cerebral cortex. In fetal tissues expressed in the cerebellum, spinal cord and cortex.
Post Translational Modifications Auto-ubiquitinated.leads to proteasomal degradation.
Function E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of SIN3B. Independently of its E3 ligase activity, acts as a CTNNB1 stabilizer through USP7-mediated deubiquitination of CTNNB1 promoting Wnt signaling. Plays a critical role in the regulation of nuclear lamina.
Protein Name E3 Ubiquitin-Protein Ligase Rnf220
Ring Finger Protein 220
Ring-Type E3 Ubiquitin Transferase Rnf220
Database Links Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rnf220 antibody
Anti-Ring Finger Protein 220 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Rnf220 antibody
Anti-RNF220 antibody
Anti-C1orf164 antibody

Information sourced from Uniprot.org

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