Anti-RNF220 antibody (267-566) (STJ118298)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RNF220 (267-566) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RNF220 |
| Gene ID | 55182 |
| Uniprot ID | RN220_HUMAN |
| Immunogen Region | 267-566 |
| Immunogen Sequence | PKSLLLSASIKREGESPTAS PHSSATDDLHHSDRYQTFLR VRANRQTRLNARIGKMKRRK QDEGQREGSCMAEDDAVDIE HENNNRFEEYEWCGQKRIRA TTLLEGGFRGSGFIMCSGKE NPDSDADLDVDGDDTLEYGK PQYTEADVIPCTGEEPGEAK EREALRGAVLNGGPPSTRIT PEFSKWASDEMPSTSNGESS KQEAMQKTCKNSDIEKITED SAVTTFEALKARVRELERQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 267-566 of human RNF220 (NP_060620.2). |
Additional Info
| Tissue Specificity | Ubiquitously expressed. Abundant in brain and spinal cord, particularly in the cerebellum and cerebral cortex. In fetal tissues expressed in the cerebellum, spinal cord and cortex. |
| Post Translational Modifications | Auto-ubiquitinated.leads to proteasomal degradation. |
| Function | E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of SIN3B. Independently of its E3 ligase activity, acts as a CTNNB1 stabilizer through USP7-mediated deubiquitination of CTNNB1 promoting Wnt signaling. Plays a critical role in the regulation of nuclear lamina. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf220Ring Finger Protein 220Ring-Type E3 Ubiquitin Transferase Rnf220 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf220 antibodyAnti-Ring Finger Protein 220 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Rnf220 antibodyAnti-RNF220 antibodyAnti-C1orf164 antibody |
Information sourced from Uniprot.org