• Western blot analysis of extracts of various cell lines, using RNF14 antibody (STJ11104109) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RNF14 antibody (300-400) [S4109RM] (STJ11104109)

SKU:
STJ11104109

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-E3 Ubiquitin-Protein Ligase Rnf14 (300-400) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4109RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNF14
Gene ID: 9604
Uniprot ID: RNF14_HUMAN
Immunogen Region: 300-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human RNF14 (Q9UBS8).
Immunogen Sequence: LDLMADVVYCPRPCCQLPVM QEPGCTMGICSSCNFAFCTL CRLTYHGVSPCKVTAEKLMD LRNEYLQADEANKRLLDQRY GKRVIQKALEEMESKEWLEK N
Tissue Specificity Widely expressed.
Post Translational Modifications RING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination.
Function E3 ubiquitin-protein ligase that plays a key role in the RNF14-RNF25 translation quality control pathway, a pathway that takes place when a ribosome has stalled during translation, and which promotes ubiquitination and degradation of translation factors on stalled ribosomes. Recruited to stalled ribosomes by the ribosome collision sensor GCN1 and mediates ubiquitination of EEF1A1/eEF1A, leading to its degradation. In addition to EEF1A1/eEF1A, RNF14 catalyzes ubiquitination of other translation factors on stalled ribosomes: mediates ubiquitination and degradation of ETF1/eRF1 and ubiquitination of ribosomal proteins RPL0, RPL1, RPL12, RPS13 and RPS17. Independently of its function in the response to stalled ribosomes, acts as a regulator of transcription in Wnt signaling via its interaction with TCF transcription factors (TCF7/TCF1, TCF7L1/TCF3 and TCF7L2/TCF4). May also play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription.
Protein Name E3 Ubiquitin-Protein Ligase Rnf14
Androgen Receptor-Associated Protein 54
Hfb30
Ring Finger Protein 14
Database Links Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rnf14 antibody
Anti-Androgen Receptor-Associated Protein 54 antibody
Anti-Hfb30 antibody
Anti-Ring Finger Protein 14 antibody
Anti-RNF14 antibody
Anti-ARA54 antibody
Anti-HRIHFB2038 antibody

Information sourced from Uniprot.org

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