Host: | Rabbit |
Applications: | IHC/WB |
Reactivity: | Human |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit polyclonal antibody anti-RNF139 (500-550 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | Lyophilised |
Purification: | IgG purified |
Dilution Range: | IHC, WB (confirmed by recombinant protein). A dilution of 1: 300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
Storage Instruction: | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Gene Symbol: | RNF139 |
Gene ID: | 11236 |
Uniprot ID: | RN139_HUMAN |
Immunogen Region: | 500-550 aa |
Specificity: | Specific for RNF139. |
Immunogen: | A synthetic peptide from aa region 500-550 of human RNF139 conjugated to an immunogenic carrier protein was used as the antigen. The peptide shares 95% identity with rat and mouse sequences. |
Post Translational Modifications | Autoubiquitinated. Ubiquitination is induced by sterol and leads to ist degradation via the ubiquitin-proteasome pathway. |
Function | E3-ubiquitin ligase.acts as a negative regulator of cell proliferation through mechanisms involving G2/M arrest and cell death. Required for MHC class I ubiquitination in cells expressing the cytomegalovirus protein US2 before dislocation from the endoplasmic reticulum (ER). Affects SREBP processing by hindering the SREBP-SCAP complex translocation from the ER to the Golgi, thereby reducing SREBF2 target gene expression. Involved in the sterol-accelerated degradation of HMGCR. This is achieved through binding of RNF139 to INSIG1 and/or INSIG2 at the ER membrane. In addition, interaction of RNF139 with AUP1 facilitates interaction of RNF139 with ubiquitin-conjugating enzyme UBE2G2 and ubiquitin ligase AMFR, leading to ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER into the cytosol for subsequent destruction. Required for INSIG1 ubiquitination. May be required for EIF3 complex ubiquitination. |
Protein Name | E3 Ubiquitin-Protein Ligase Rnf139 Ring Finger Protein 139 Ring-Type E3 Ubiquitin Transferase Rnf139 Translocation In Renal Carcinoma On Chromosome 8 Protein |
Database Links | Reactome: R-HSA-901032 |
Cellular Localisation | Endoplasmic Reticulum Membrane Multi-Pass Membrane Protein |
Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf139 antibody Anti-Ring Finger Protein 139 antibody Anti-Ring-Type E3 Ubiquitin Transferase Rnf139 antibody Anti-Translocation In Renal Carcinoma On Chromosome 8 Protein antibody Anti-RNF139 antibody Anti-TRC8 antibody |
Information sourced from Uniprot.org