| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RNF125 (41-232) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RNF125 |
| Gene ID: |
54941 |
| Uniprot ID: |
RN125_HUMAN |
| Immunogen Region: |
41-232 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 41-232 of human RNF125 (NP_060301.2). |
| Immunogen Sequence: |
LEVLHQPVRTRCGHVFCRSC IATSLKNNKWTCPYCRAYLP SEGVPATDVAKRMKSEYKNC AECDTLVCLSEMRAHIRTCQ KYIDKYGPLQELEETAARCV CPFCQRELYEDSLLDHCITH HRSERRPVFCPLCRLIPDEN PSSFSGSLIRHLQVSHTLFY |
| Tissue Specificity | Predominantly expressed in lymphoid tissues, including bone marrow, spleen and thymus. Also weakly expressed in other tissues. Predominant in the CD4(+) and CD8(+) T-cells, suggesting that it is preferentially confined to T-cells. |
| Post Translational Modifications | Autoubiquitinated, leading to its subsequent proteasomal degradation. |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins, such as RIGI, MAVS/IPS1, IFIH1/MDA5, JAK1 and p53/TP53. Acts as a negative regulator of type I interferon production by mediating ubiquitination of RIGI at 'Lys-181', leading to RIGI degradation. Mediates ubiquitination and subsequent degradation of p53/TP53. Mediates ubiquitination and subsequent degradation of JAK1. Acts as a positive regulator of T-cell activation. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf125Ring Finger Protein 125T-Cell Ring Activation Protein 1Trac-1 |
| Database Links | Reactome: R-HSA-936440 |
| Cellular Localisation | Golgi Apparatus MembraneLipid-AnchorShows A Reticular Staining Pattern Within The Cell And Is Probably Expressed At Other Intracellular Membranes In Addition To The Golgi MembraneNot Detected At The Plasma Membrane |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf125 antibodyAnti-Ring Finger Protein 125 antibodyAnti-T-Cell Ring Activation Protein 1 antibodyAnti-Trac-1 antibodyAnti-RNF125 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
