• Western blot analysis of various lysates, using RNF125 Rabbit polyclonal antibody (STJ117360) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of various lysates using RNF125 Rabbit polyclonal antibody (STJ117360) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-RNF125 antibody (1-105) (STJ117360)

SKU:
STJ117360

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RNF125 (1-105) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNF125
Gene ID: 54941
Uniprot ID: RN125_HUMAN
Immunogen Region: 1-105
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human RNF125 (NP_060301.2).
Immunogen Sequence: MGSVLSTDSGKSAPASATAR ALERRRDPELPVTSFDCAVC LEVLHQPVRTRCGHVFCRSC IATSLKNNKWTCPYCRAYLP SEGVPATDVAKRMKSEYKNC AECDT
Tissue Specificity Predominantly expressed in lymphoid tissues, including bone marrow, spleen and thymus. Also weakly expressed in other tissues. Predominant in the CD4(+) and CD8(+) T-cells, suggesting that it is preferentially confined to T-cells.
Post Translational Modifications Autoubiquitinated, leading to its subsequent proteasomal degradation.
Function E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins, such as RIGI, MAVS/IPS1, IFIH1/MDA5, JAK1 and p53/TP53. Acts as a negative regulator of type I interferon production by mediating ubiquitination of RIGI at 'Lys-181', leading to RIGI degradation. Mediates ubiquitination and subsequent degradation of p53/TP53. Mediates ubiquitination and subsequent degradation of JAK1. Acts as a positive regulator of T-cell activation.
Protein Name E3 Ubiquitin-Protein Ligase Rnf125
Ring Finger Protein 125
T-Cell Ring Activation Protein 1
Trac-1
Database Links Reactome: R-HSA-936440
Cellular Localisation Golgi Apparatus Membrane
Lipid-Anchor
Shows A Reticular Staining Pattern Within The Cell And Is Probably Expressed At Other Intracellular Membranes In Addition To The Golgi Membrane
Not Detected At The Plasma Membrane
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rnf125 antibody
Anti-Ring Finger Protein 125 antibody
Anti-T-Cell Ring Activation Protein 1 antibody
Anti-Trac-1 antibody
Anti-RNF125 antibody

Information sourced from Uniprot.org

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