Western blot analysis of various lysates using RNASE2 Rabbit pAb (STJ111990) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-RNASE2 antibody (28-161) (STJ111990)

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STJ111990
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNASE2
Gene ID: 6036
Uniprot ID: RNAS2_HUMAN
Immunogen Region: 28-161
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 28-161 of human RNASE2 (NP_002925.1).
Immunogen Sequence: KPPQFTWAQWFETQHINMTS QQCTNAMQVINNYQRRCKNQ NTFLLTTFANVVNVCGNPNM TCPSNKTRKNCHHSGSQVPL IHCNLTTPSPQNISNCRYAQ TPANMFYIVACDNRDQRRDP PQYPVVPVHLDRII
Tissue Specificity Liver, lung, spleen, leukocytes and body fluids.
Post Translational Modifications A particular signal processing and glycosylation pattern may differentiate the UpI2 RNase, found specifically in pregnant women urine, from other nonsecretory RNases.
Function This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Selectively chemotactic for dendritic cells. Possesses a wide variety of biological activities.
Protein Name Non-Secretory Ribonuclease
Eosinophil-Derived Neurotoxin
Rnase Upi-2
Ribonuclease 2
Rnase 2
Ribonuclease Us
Database Links Reactome: R-HSA-6798695
Cellular Localisation Lysosome
Cytoplasmic Granule
Matrix Of Eosinophil's Large Specific Granule
Alternative Antibody Names Anti-Non-Secretory Ribonuclease antibody
Anti-Eosinophil-Derived Neurotoxin antibody
Anti-Rnase Upi-2 antibody
Anti-Ribonuclease 2 antibody
Anti-Rnase 2 antibody
Anti-Ribonuclease Us antibody
Anti-RNASE2 antibody
Anti-EDN antibody
Anti-RNS2 antibody

Information sourced from Uniprot.org

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