Anti-RHOU antibody (40-100) (STJ119338)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ119338
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General Information

Short DescriptionRabbit polyclonal RHOU (40-100) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolRHOU
Gene ID58480
Uniprot IDRHOU_HUMAN
Immunogen Region40-100
Immunogen SequenceGRAGGAEGRGVKCVLVGDGA VGKTSLVVSYTTNGYPTEYI PTAFDNFSAVVSVDGRPVRL Q
SpecificityA synthetic peptide corresponding to a sequence within amino acids 40-100 of human RHOU (NP_067028.1).

Additional Info

Tissue Specificity Ubiquitously expressed in all tissues examined. Expressed at high levels in the stomach, small intestine, brain, skeletal muscle and placenta.
Post Translational Modifications Ubiquitinated. 'Lys-48'-linked ubiquitination at Lys-177 and Lys-248 by the ECS(RAB40A) complex leading to its degradation. Tyrosine phosphorylated by SRC in response to PTK2B/PYK2 activation.
Function Binds to and activates protein kinase PAK1. Plays a role in the regulation of cell morphology, cytoskeletal organization and focal adhesion assembly during cell migration. Also stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound.
Protein Name Rho-Related Gtp-Binding Protein Rhou
Cdc42-Like Gtpase 1
Gtp-Binding Protein-Like 1
Rho Gtpase-Like Protein Arhu
Ryu Gtpase
Wnt-1 Responsive Cdc42 Homolog 1
Wrch-1
Database Links Reactome: R-HSA-6785807
Reactome: R-HSA-9013420
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Golgi Apparatus Membrane
Cell Junction
Focal Adhesion
Cell Projection
Podosome
Localizes To Podosomes In Src-Transformed Cells
Alternative Antibody Names Anti-Rho-Related Gtp-Binding Protein Rhou antibody
Anti-Cdc42-Like Gtpase 1 antibody
Anti-Gtp-Binding Protein-Like 1 antibody
Anti-Rho Gtpase-Like Protein Arhu antibody
Anti-Ryu Gtpase antibody
Anti-Wnt-1 Responsive Cdc42 Homolog 1 antibody
Anti-Wrch-1 antibody
Anti-RHOU antibody
Anti-ARHU antibody
Anti-CDC42L1 antibody
Anti-G28K antibody
Anti-WRCH1 antibody
Anti-SB128 antibody

Information sourced from Uniprot.org

Citations

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