![Western blot analysis of various lysates using [KD Validated] RHOT1/MIRO1 Rabbit pAb (STJ28401) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101691/411334/STJ28401_1__58114.1744897868.jpg?c=1 "Western blot analysis of various lysates using [KD Validated] RHOT1/MIRO1 Rabbit pAb (STJ28401) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
![Western blot analysis of various lysates using [KD Validated] RHOT1/MIRO1 Rabbit pAb (STJ28401) at 1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101691/411335/STJ28401_1__12027.1744897868.jpg?c=1 "Western blot analysis of various lysates using [KD Validated] RHOT1/MIRO1 Rabbit pAb (STJ28401) at 1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and RHOT1/MIRO1 knockdown (KD) 293T cells using [KD Validated] RHOT1/MIRO1 Rabbit pAb (STJ28401) at 1:5000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101691/411336/STJ28401_2__22198.1744897869.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and RHOT1/MIRO1 knockdown (KD) 293T cells using [KD Validated] RHOT1/MIRO1 Rabbit pAb (STJ28401) at 1:5000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.")
![Western blot analysis of lysates from Mouse testis using [KD Validated] RHOT1/MIRO1 Rabbit pAb (STJ28401) at 1:5000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101691/411337/STJ28401_3__87909.1744897870.jpg?c=1 "Western blot analysis of lysates from Mouse testis using [KD Validated] RHOT1/MIRO1 Rabbit pAb (STJ28401) at 1:5000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.")
![Western blot analysis of various lysates using [KD Validated] RHOT1/MIRO1 Rabbit pAb (STJ28401) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/101691/411334/STJ28401_1__58114.1744897868.jpg?c=1 "Western blot analysis of various lysates using [KD Validated] RHOT1/MIRO1 Rabbit pAb (STJ28401) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
Anti-RHOT1 antibody (310-590) (STJ28401)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RHOT1 (310-590) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:2000-1:6000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RHOT1 |
| Gene ID | 55288 |
| Uniprot ID | MIRO1_HUMAN |
| Immunogen Region | 310-590 |
| Immunogen Sequence | QSTFDKHDLDRDCALSPDEL KDLFKVFPYIPWGPDVNNTV CTNERGWITYQGFLSQWTLT TYLDVQRCLEYLGYLGYSIL TEQESQASAVTVTRDKKIDL QKKQTQRNVFRCNVIGVKNC GKSGVLQALLGRNLMRQKKI REDHKSYYAINTVYVYGQEK YLLLHDISESEFLTEAEIIC DVVCLVYDVSNPKSFEYCAR IFKQHFMDSRIPCLIVAAKS DLHEVKQEYSISPTDFCRK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 310-590 of human RHOT1/MIRO1 (NP_060777.3). |
Additional Info
| Tissue Specificity | Ubiquitously expressed. Expressed at high level in heart and skeletal muscle. |
| Post Translational Modifications | Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30. Acetylation on Lys-92 decreases sensitivity of mitochondrial transport to elevated Ca(2+) levels, increases mitochondrial transport and promotes axon growth. Deacetylated by HDAC6 which blocks mitochondrial transport and mediates axon growth inhibition. |
| Function | Atypical mitochondrial nucleoside-triphosphatase (NTPase) involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution. Promotes mitochondrial fission during high calcium conditions. Can hydrolyze GTP, ATP and UTP. |
| Protein Name | Mitochondrial Rho Gtpase 1Miro-1Hmiro-1Rac-Gtp-Binding Protein-Like ProteinRas Homolog Gene Family Member T1 |
| Database Links | Reactome: R-HSA-5689880Reactome: R-HSA-9013425 |
| Cellular Localisation | Mitochondrion Outer MembraneSingle-Pass Type Iv Membrane ProteinColocalizes With Mgarp And Rhot2 At The Mitochondria |
| Alternative Antibody Names | Anti-Mitochondrial Rho Gtpase 1 antibodyAnti-Miro-1 antibodyAnti-Hmiro-1 antibodyAnti-Rac-Gtp-Binding Protein-Like Protein antibodyAnti-Ras Homolog Gene Family Member T1 antibodyAnti-RHOT1 antibodyAnti-ARHT1 antibody |
Information sourced from Uniprot.org