![Immunofluorescence analysis of HeLa cells using [KD Validated] RHOT1/MIRO1 Rabbit polyclonal antibody (STJ11104873) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177517/369319/STJ11104873_1__63712.1713134807.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KD Validated] RHOT1/MIRO1 Rabbit polyclonal antibody (STJ11104873) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of HeLa cells using [KD Validated] RHOT1/MIRO1 Rabbit polyclonal antibody (STJ11104873) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/177517/369319/STJ11104873_1__63712.1713134807.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KD Validated] RHOT1/MIRO1 Rabbit polyclonal antibody (STJ11104873) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.")
| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:100-1:500IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RHOT1 |
| Gene ID: | 55288 |
| Uniprot ID: | MIRO1_HUMAN |
| Immunogen Region: | 310-590 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 310-590 of human RHOT1/MIRO1. (NP_060777.3). |
| Immunogen Sequence: | QSTFDKHDLDRDCALSPDEL KDLFKVFPYIPWGPDVNNTV CTNERGWITYQGFLSQWTLT TYLDVQRCLEYLGYLGYSIL TEQESQASAVTVTRDKKIDL QKKQTQRNVFRCNVIGVKNC GKSGVLQALLGRNLMRQKKI REDHKSYYAINTVYVYGQEK YLLLHDISESEFLTEAEIIC DVVCLVYDVSNPKSFEYCAR IFKQHFMDSRIPCLIVAAKS DLHEVKQEYSISPTDFCRK |
| Tissue Specificity | Ubiquitously expressed. Expressed at high level in heart and skeletal muscle. |
| Post Translational Modifications | Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30. |
| Function | Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution. Promotes mitochondrial fission during high calcium conditions. |
| Protein Name | Mitochondrial Rho Gtpase 1Miro-1Hmiro-1Rac-Gtp-Binding Protein-Like ProteinRas Homolog Gene Family Member T1 |
| Database Links | Reactome: R-HSA-5689880Reactome: R-HSA-9013425 |
| Cellular Localisation | Mitochondrion Outer MembraneSingle-Pass Type Iv Membrane ProteinColocalizes With Mgarp And Rhot2 At The Mitochondria |
| Alternative Antibody Names | Anti-Mitochondrial Rho Gtpase 1 antibodyAnti-Miro-1 antibodyAnti-Hmiro-1 antibodyAnti-Rac-Gtp-Binding Protein-Like Protein antibodyAnti-Ras Homolog Gene Family Member T1 antibodyAnti-RHOT1 antibodyAnti-ARHT1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of various lysates using [KD Validated] RHOT1/MIRO1 Rabbit pAb (STJ28401) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/101691/411334/STJ28401_1__58114.1744897868.jpg?c=1 "Western blot analysis of various lysates using [KD Validated] RHOT1/MIRO1 Rabbit pAb (STJ28401) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
