• Immunofluorescence analysis of HeLa cells using [KD Validated] RHOT1/MIRO1 Rabbit polyclonal antibody (STJ11104873) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts from wild type (WT) and RHOT1/MIRO1 knockdown (KD) 293T (KD) cells, using RHOT1/MIRO1 antibody (STJ11104873) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of Rat testis, using RHOT1/MIRO1 antibody (STJ11104873) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of various lysates, using RHOT1/MIRO1 antibody (STJ11104873) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RHOT1 antibody (310-590) (STJ11104873)

SKU:
STJ11104873

€161.88 - €799.38
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Mitochondrial Rho Gtpase 1 (310-590) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RHOT1
Gene ID: 55288
Uniprot ID: MIRO1_HUMAN
Immunogen Region: 310-590
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 310-590 of human RHOT1/MIRO1. (NP_060777.3).
Immunogen Sequence: QSTFDKHDLDRDCALSPDEL KDLFKVFPYIPWGPDVNNTV CTNERGWITYQGFLSQWTLT TYLDVQRCLEYLGYLGYSIL TEQESQASAVTVTRDKKIDL QKKQTQRNVFRCNVIGVKNC GKSGVLQALLGRNLMRQKKI REDHKSYYAINTVYVYGQEK YLLLHDISESEFLTEAEIIC DVVCLVYDVSNPKSFEYCAR IFKQHFMDSRIPCLIVAAKS DLHEVKQEYSISPTDFCRK
Tissue Specificity Ubiquitously expressed. Expressed at high level in heart and skeletal muscle.
Post Translational Modifications Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.
Function Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution. Promotes mitochondrial fission during high calcium conditions.
Protein Name Mitochondrial Rho Gtpase 1
Miro-1
Hmiro-1
Rac-Gtp-Binding Protein-Like Protein
Ras Homolog Gene Family Member T1
Database Links Reactome: R-HSA-5689880
Reactome: R-HSA-9013425
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Type Iv Membrane Protein
Colocalizes With Mgarp And Rhot2 At The Mitochondria
Alternative Antibody Names Anti-Mitochondrial Rho Gtpase 1 antibody
Anti-Miro-1 antibody
Anti-Hmiro-1 antibody
Anti-Rac-Gtp-Binding Protein-Like Protein antibody
Anti-Ras Homolog Gene Family Member T1 antibody
Anti-RHOT1 antibody
Anti-ARHT1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance