• Immunohistochemistry analysis of Rhodopsin in paraffin-embedded mouse retina using Rhodopsin Rabbit polyclonal antibody (STJ117287) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Rhodopsin Rabbit polyclonal antibody (STJ117287) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RHO antibody (C-Term) (STJ117287)

SKU:
STJ117287

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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RHO (C-Term) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RHO
Gene ID: 6010
Uniprot ID: OPSD_HUMAN
Immunogen Region: C-Term
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human Rhodopsindopsin (NP_000530.1).
Immunogen Sequence: NESFVIYMFVVHFTIPMIII FFCYGQLVFTVKEAAAQQQE SATTQKAEKEVTRMVIIMVI AFLICWVPYASVAFYIFTHQ GSNFGPIFMTIPAFFAKSAA IYNPVIYIMMNKQFRNCMLT TICCGKNPLGDDEASATVSK TETSQVAPA
Tissue Specificity Rod shaped photoreceptor cells which mediate vision in dim light.
Post Translational Modifications Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region. After activation by light, phosphorylated by GRK1 (in vitro). Contains one covalently linked retinal chromophore. Upon light absorption, the covalently bound 11-cis-retinal is converted to all-trans-retinal. After hydrolysis of the Schiff base and release of the covalently bound all-trans-retinal, active rhodopsin is regenerated by binding of a fresh molecule of 11-cis-retinal.
Function Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling.
Protein Name Rhodopsin
Opsin-2
Database Links Reactome: R-HSA-2453902
Reactome: R-HSA-2485179
Reactome: R-HSA-2514859
Reactome: R-HSA-418594
Reactome: R-HSA-419771
Reactome: R-HSA-5620916
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Cell Projection
Cilium
Photoreceptor Outer Segment
Synthesized In The Inner Segment (Is) Of Rod Photoreceptor Cells Before Vectorial Transport To Disk Membranes In The Rod Outer Segment (Os) Photosensory Cilia
Alternative Antibody Names Anti-Rhodopsin antibody
Anti-Opsin-2 antibody
Anti-RHO antibody
Anti-OPN2 antibody

Information sourced from Uniprot.org

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