Anti-RFP antibody (STJ140119)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
ImmunogenPurified recombinant RFP peptide produced in E. coli.
General Information
| Short Description | Goat polyclonal Red Fluorescent Protein antibody for use in WB, IF, IHC-P, IHC-F and IEM in rfp, dsred, mcherry, morange, mplum, mrfp, mstrawberry and tdtomato samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/IHC-P/IHC-F/IEM |
| Host | Goat |
| Reactivity | RFP/DsRed/mCherry/mOrange/mPlum/mRFP/mStrawberry/tdTomato |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 3 mg/mL |
| Purification | This antibody is epitope-affinity purified from goat antiserum. |
| Dilution Range | WB 1:500-1:2000IHC-F 1:50-1:500IHC-P 1:50-1:500IF 1:50-1:500ELISA NDTan Z Kong M Wen S et al. J Bone Miner Res 2020 Jul. PMID: 32662900Endo T Kadoya K Suzuki Y et al. Front Cell Neurosci 2019 Jun. PMID: 31316351Jian |
| Formulation | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Immunogen | Purified recombinant RFP peptide produced in E. coli. |
| Immunogen Sequence | MVSKGEEDNMAIIKEFMRFK VSKGEEDNMAIIKEFMRFKV SKGEEDNMAIIKEFMRFKVH KGEEDNMAIIKEFMRFKVHM GEEDNMAIIKEFMRFKVHME EEDNMAIIKEFMRFKVHMEG EDNMAIIKEFMRFKVHMEGS DNMAIIKEFMRFKVHMEGSV NMAIIKEFMRFKVHMEGSVN MAIIKEFMRFKVHMEGSVNG AIIKEFMRFKVHMEGSVNGH IIKEFMRFKVHMEGSVNGH |
| Specificity | In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody also recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein). |
Additional Info
| Background | Goat polyclonal antibody to RFP (Red fluorescent protein). RFP is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. |
Information sourced from Uniprot.org