| Short Description : | Goat polyclonal Red Fluorescent Protein antibody for use in WB, IF, IHC-P, IHC-F and IEM in mcherry, tdtomato and rfp samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/IHC-P/IHC-F/IEM |
| Host: | Goat |
| Reactivity: | mCherry/tdTomato/RFP |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 3 mg/mL |
| Purification: | This antibody is epitope-affinity purified from goat antiserum. |
| Dilution Range: | WB 1:500-1:5000IF 1:50-1:500IHC-P 1:50-1:500IHC-F 1:50-1:500IEM 1:50-1:500Steenbergen VV Burattini L Trumpp M et al. J Exp Med 2023 PMID: 36571760Stewart S Le Bleu HK Yette GA et al. bioRxiv Oct 2019Déglon N Merienn |
| Formulation: | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction: | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Immunogen: | Purified recombinant peptide produced in E. coli. |
| Immunogen Sequence: | MVSKGEEDNMAIIKEFMRFK VSKGEEDNMAIIKEFMRFKV SKGEEDNMAIIKEFMRFKVH KGEEDNMAIIKEFMRFKVHM GEEDNMAIIKEFMRFKVHME EEDNMAIIKEFMRFKVHMEG EDNMAIIKEFMRFKVHMEGS DNMAIIKEFMRFKVHMEGSV NMAIIKEFMRFKVHMEGSVN MAIIKEFMRFKVHMEGSVNG AIIKEFMRFKVHMEGSVNGH IIKEFMRFKVHMEGSVNGH |
| Specificity: | In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein). |
| Background | Goat polyclonal antibody to mCherry (Cherry fluorescent protein). mCherry protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. |
Information sourced from Uniprot.org
