| Host: | Goat |
| Applications: | WB/IHC-F/IHC-P/IF/IEM |
| Reactivity: | mCherry/tdTomato/RFP |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: | This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: | 3 mg/mL |
| Dilution Range: | WB 1:500-1:5000IF 1:50-1:500IHC-P 1:50-1:500IHC-F 1:50-1:500IEM 1:50-1:500Takahashi TM Hirano A Kanda T et al. Cell Rep Methods 2022 Nov PMID: 36452866MacDonald AJ Holmes FE Beall C et al. bioRxiv 2019Al-Osta I Much |
| Storage Instruction: | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Specificity: | In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody recognizes very well tdTomato and does not recognize GFP (green fluorescent protein). |
| Immunogen: | Purified recombinant peptide produced in E. coli. |
| Immunogen Sequence: | MTMITPSLHACRSTLEDPRV PVATMVSKGEEDNMAIIKEF MRFKVHMEGSVNGHEFEIEG EGEGRPYEGTQTAKLKVTKG GPLPFAWDILSPQFMYGSKA YVKHPADIPDYLKLSFPEGF KWERVMNFEDGGVVTVTQDS SLQDGEFIYKVKLRGTNFPS DGPVMQKKTMGWEASSERMY PEDGALKGEIKQRLKLKDGG HYDAEVKTTYKAKKPVQLPG AYNVNIKLDITSHNEDYTI |
| Background | Goat polyclonal antibody to mCherry (Cherry fluorescent protein). |
Information sourced from Uniprot.org