• Western blot analysis of extracts of various cell lines, using RFC2 antibody (STJ28929) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-RFC2 antibody (38-297) (STJ28929)

SKU:
STJ28929

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RFC2 (38-297) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RFC2
Gene ID: 5982
Uniprot ID: RFC2_HUMAN
Immunogen Region: 38-297
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 38-297 of human RFC2 (NP_852136.1).
Immunogen Sequence: WVEKYRPVKLNEIVGNEDTV SRLEVFAREGNVPNIIIAGP PGTGKTTSILCLARALLGPA LKDAMLELNASNDRGIDVVR NKIKMFAQQKVTLPKGRHKI IILDEADSMTDGAQQALRRT MEIYSKTTRFALACNASDKI IEPIQSRCAVLRYTKLTDAQ ILTRLMNVIEKERVPYTDDG LEAIIFTAQGDMRQALNNLQ STFSGFGFINSENVFKVCDE PHPLLVKEMIQHCVNANID
Function The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP.
Protein Name Replication Factor C Subunit 2
Activator 1 40 Kda Subunit
A1 40 Kda Subunit
Activator 1 Subunit 2
Replication Factor C 40 Kda Subunit
Rf-C 40 Kda Subunit
Rfc40
Database Links Reactome: R-HSA-110312
Reactome: R-HSA-110314
Reactome: R-HSA-110320
Reactome: R-HSA-174411
Reactome: R-HSA-176187
Reactome: R-HSA-5651801
Reactome: R-HSA-5655862
Reactome: R-HSA-5656121
Reactome: R-HSA-5656169
Reactome: R-HSA-5685938
Reactome: R-HSA-5685942
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-5696397
Reactome: R-HSA-5696400
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6804756
Reactome: R-HSA-69091
Reactome: R-HSA-69473
Reactome: R-HSA-9709570
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Replication Factor C Subunit 2 antibody
Anti-Activator 1 40 Kda Subunit antibody
Anti-A1 40 Kda Subunit antibody
Anti-Activator 1 Subunit 2 antibody
Anti-Replication Factor C 40 Kda Subunit antibody
Anti-Rf-C 40 Kda Subunit antibody
Anti-Rfc40 antibody
Anti-RFC2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance