| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RFC1 |
| Gene ID: | 5981 |
| Uniprot ID: | RFC1_HUMAN |
| Immunogen Region: | 400-700 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 400-700 of human RFC1 (NP_001191676.1). |
| Immunogen Sequence: | PKGAENCLEGLIFVITGVLE SIERDEAKSLIERYGGKVTG NVSKKTNYLVMGRDSGQSKS DKAAALGTKIIDEDGLLNLI RTMPGKKSKYEIAVETEMKK ESKLERTPQKNVQGKRKISP SKKESESKKSRPTSKRDSLA KTIKKETDVFWKSLDFKEQV AEETSGDSKARNLADDSSEN KVENLLWVDKYKPTSLKTII GQQGDQSCANKLLRWLRNWQ KSSSEDKKHAAKFGKFSGK |
| Tissue Specificity | Wide tissue distribution. Undetectable in placental tissue. |
| Function | The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA. Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair. |
| Protein Name | Replication Factor C Subunit 1 Activator 1 140 Kda Subunit A1 140 Kda Subunit Activator 1 Large Subunit Activator 1 Subunit 1 Dna-Binding Protein Po-Ga Replication Factor C 140 Kda Subunit Rf-C 140 Kda Subunit Rfc140 Replication Factor C Large Subunit |
| Database Links | Reactome: R-HSA-110312 Reactome: R-HSA-110314 Reactome: R-HSA-110320 Reactome: R-HSA-174411 Reactome: R-HSA-5651801 Reactome: R-HSA-5655862 Reactome: R-HSA-5656121 Reactome: R-HSA-5656169 Reactome: R-HSA-5685942 Reactome: R-HSA-5696397 Reactome: R-HSA-5696400 Reactome: R-HSA-6782135 Reactome: R-HSA-6782210 Reactome: R-HSA-69091 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Replication Factor C Subunit 1 antibody Anti-Activator 1 140 Kda Subunit antibody Anti-A1 140 Kda Subunit antibody Anti-Activator 1 Large Subunit antibody Anti-Activator 1 Subunit 1 antibody Anti-Dna-Binding Protein Po-Ga antibody Anti-Replication Factor C 140 Kda Subunit antibody Anti-Rf-C 140 Kda Subunit antibody Anti-Rfc140 antibody Anti-Replication Factor C Large Subunit antibody Anti-RFC1 antibody Anti-RFC140 antibody |
Information sourced from Uniprot.org