| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RFC1 (400-700) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RFC1 |
| Gene ID: |
5981 |
| Uniprot ID: |
RFC1_HUMAN |
| Immunogen Region: |
400-700 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 400-700 of human RFC1 (NP_001191676.1). |
| Immunogen Sequence: |
PKGAENCLEGLIFVITGVLE SIERDEAKSLIERYGGKVTG NVSKKTNYLVMGRDSGQSKS DKAAALGTKIIDEDGLLNLI RTMPGKKSKYEIAVETEMKK ESKLERTPQKNVQGKRKISP SKKESESKKSRPTSKRDSLA KTIKKETDVFWKSLDFKEQV AEETSGDSKARNLADDSSEN KVENLLWVDKYKPTSLKTII GQQGDQSCANKLLRWLRNWQ KSSSEDKKHAAKFGKFSGK |
| Tissue Specificity | Wide tissue distribution. Undetectable in placental tissue. |
| Function | The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA. Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair. |
| Protein Name | Replication Factor C Subunit 1Activator 1 140 Kda SubunitA1 140 Kda SubunitActivator 1 Large SubunitActivator 1 Subunit 1Dna-Binding Protein Po-GaReplication Factor C 140 Kda SubunitRf-C 140 Kda SubunitRfc140Replication Factor C Large Subunit |
| Database Links | Reactome: R-HSA-110312Reactome: R-HSA-110314Reactome: R-HSA-110320Reactome: R-HSA-174411Reactome: R-HSA-5651801Reactome: R-HSA-5655862Reactome: R-HSA-5656121Reactome: R-HSA-5656169Reactome: R-HSA-5685942Reactome: R-HSA-5696397Reactome: R-HSA-5696400Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-69091 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Replication Factor C Subunit 1 antibodyAnti-Activator 1 140 Kda Subunit antibodyAnti-A1 140 Kda Subunit antibodyAnti-Activator 1 Large Subunit antibodyAnti-Activator 1 Subunit 1 antibodyAnti-Dna-Binding Protein Po-Ga antibodyAnti-Replication Factor C 140 Kda Subunit antibodyAnti-Rf-C 140 Kda Subunit antibodyAnti-Rfc140 antibodyAnti-Replication Factor C Large Subunit antibodyAnti-RFC1 antibodyAnti-RFC140 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and HERC4 knockout (KO) HeLa cells, using [KO Validated] HERC4 Rabbit polyclonal antibody (STJ11100022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89094/357553/STJ11100022_1__83889.1713121675.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HERC4 knockout (KO) HeLa cells, using [KO Validated] HERC4 Rabbit polyclonal antibody (STJ11100022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")