Anti-Recombinant-MANF antibody [3D4] (STJA0002919)

Anti-Recombinant-MANF antibody [3D4] (STJA0002919)

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STJA0002919
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Host: Mouse
Applications: ELISA/IF/IHC-C
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Mouse monoclonal anti-Recombinant-MANF for use in ELISA, IF and IHC-C in Human samples. Datasheet included with dilution recommendations, and related reagents.
Clonality : Monoclonal
Clone ID : 3D4
Conjugation: Unconjugated
Isotype: IgG2a
Formulation: PBS pH 7.4, with 0.1% sodium azide
Purification: MabSelect affinity chromatography
Dilution Range: ELISA 0.02 to 0.1 µg/ml; IF 1 to 20 µg/ml; IHC-C 5 µg/ml. Conformational antibody, not suitable for Western Blot application. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
Storage Instruction: Store at-20 to-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
Gene Symbol: MANF
Gene ID: 7873
Uniprot ID: MANF_HUMAN
Specificity: Human MANF
Immunogen: Recombinant human MANF protein produced using CHO-based proprietary suspension cell line. Immunogen is purified from cell culture supernatant. Human MANF
Post Translational Modifications May contain sialic acid residues.
Function Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions. Stabilizes HSPA5/BiP in its substrate-bound ADP state, which facilitates HSPA5/BiP incorporation into chaperone-client complexes during endoplasmic reticulum stress, its interaction with HSPA5/BiP inhibits ATP binding to HSPA5/BiP and subsequent nucleotide exchange. As a result acts as a repressor of the unfolded protein response (UPR) pathway. Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia. Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells. Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions. Essential for embryonic lung development. Required for the correct postnatal temporal and structural development of splenic white pulp. Required for the repair-associated myeloid response in skeletal muscle, acts as a regulator of phenotypic transition towards prorepair macrophages in response to muscle injury and as a result limits excessive proinflammatory signaling. Represses RELA expression and therefore NF-kB signaling in the myocardium, as a result limits macrophage infiltration of injured tissue and M1 macrophage differentiation in response to myocardial injury. Required for endochondral ossification in long bones and the skull during postnatal development.
Protein Name Mesencephalic Astrocyte-Derived Neurotrophic Factor
Arginine-Rich Protein
Protein Armet
Database Links Reactome: R-HSA-114608
Cellular Localisation Secreted
Endoplasmic Reticulum Lumen
Sarcoplasmic Reticulum Lumen
Retained In The Endoplasmic Reticulum (Er)
And Sarcoplasmic Reticulum (Sr) Under Normal Conditions
Up-Regulated And Secreted By The Er/Sr In Response To Er Stress And Hypoxia
Alternative Antibody Names Anti-Mesencephalic Astrocyte-Derived Neurotrophic Factor antibody
Anti-Arginine-Rich Protein antibody
Anti-Protein Armet antibody
Anti-MANF antibody
Anti-ARMET antibody
Anti-ARP antibody

Information sourced from Uniprot.org

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