• Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using CoREST/RCOR1 antibody (STJ11101902) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunoprecipitation analysis of 300 Mu g extracts of THP-1 cells using 3 Mu g CoREST/RCOR1 antibody (STJ11101902). Western blot was performed from the immunoprecipitate using CoREST/RCOR1 antibody (STJ11101902) at a dilution of 1:1000.
  • Immunohistochemistry analysis of CoREST/RCOR1 in paraffin-embedded rat colon tissue using CoREST/RCOR1 Rabbit monoclonal antibody (STJ11101902) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CoREST/RCOR1 in paraffin-embedded mouse colon tissue using CoREST/RCOR1 Rabbit monoclonal antibody (STJ11101902) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CoREST/RCOR1 in paraffin-embedded Human lung adenocarcinoma tissue using CoREST/RCOR1 Rabbit monoclonal antibody (STJ11101902) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from C2C12 cells, using CoREST/RCOR1 Rabbit monoclonal antibody (STJ11101902) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Western blot analysis of various lysates using CoREST/RCOR1 Rabbit monoclonal antibody (STJ11101902) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-RCOR1 antibody (1-100) [S2MR] (STJ11101902)

SKU:
STJ11101902

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IHC-P/IP/ELISA/ChIP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:200-1:800
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RCOR1
Gene ID: 23186
Uniprot ID: RCOR1_HUMAN
Immunogen Region: 1-100
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CoREST/RCOR1 (Q9UKL0).
Immunogen Sequence: MPAMVEKGPEVSGKRRGRNN AAASASAAAASAAASAACAS PAATAASGAAASSASAAAAS AAAAPNNGQNKSLAAAAPNG NSSSNSWEEGSSGSSSDEEH
Tissue Specificity Ubiquitously expressed.
Post Translational Modifications Phosphorylated by HSV-1 protein kinases in case of infection.
Function Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates. It also protects KDM1A from the proteasome. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation.
Protein Name Rest Corepressor 1
Protein Corest
Database Links Reactome: R-HSA-3214815
Reactome: R-HSA-8943724
Reactome: R-HSA-9679191
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Upon Infection By Hsv-1
It Is Partially Translocated Into The Cytoplasm In An Hsv-1-Dependent Manner
Alternative Antibody Names Anti-Rest Corepressor 1 antibody
Anti-Protein Corest antibody
Anti-RCOR1 antibody
Anti-KIAA0071 antibody
Anti-RCOR antibody

Information sourced from Uniprot.org

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