![Western blot analysis of lysates from wild type (WT) and RCN2 knockout (KO) 293T cells, using [KO Validated] RCN2 Rabbit polyclonal antibody (STJ11100819) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89755/358231/STJ11100819_1__07481.1713122561.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and RCN2 knockout (KO) 293T cells, using [KO Validated] RCN2 Rabbit polyclonal antibody (STJ11100819) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and RCN2 knockout (KO) 293T cells, using [KO Validated] RCN2 Rabbit polyclonal antibody (STJ11100819) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89755/358231/STJ11100819_1__07481.1713122561.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and RCN2 knockout (KO) 293T cells, using [KO Validated] RCN2 Rabbit polyclonal antibody (STJ11100819) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-RCN2 antibody (23-180) (STJ11100819)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RCN2 (23-180) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RCN2 |
| Gene ID | 5955 |
| Uniprot ID | RCN2_HUMAN |
| Immunogen Region | 23-180 |
| Immunogen Sequence | GKAEELHYPLGERRSDYDRE ALLGVQEDVDEYVKLGHEEQ QKRLQAIIKKIDLDSDGFLT ESELSSWIQMSFKHYAMQEA KQQFVEYDKNSDDTVTWDEY NIQMYDRVIDFDENTALDDA EEESFRKLHLKDKKRFEKAN QDSGPGLSLEEFIAFEHP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 23-180 of human RCN2 (NP_002893.1). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Function | Not known. Binds calcium. |
| Protein Name | Reticulocalbin-2Calcium-Binding Protein Erc-55E6-Binding ProteinE6bp |
| Database Links | |
| Cellular Localisation | Endoplasmic Reticulum Lumen |
| Alternative Antibody Names | Anti-Reticulocalbin-2 antibodyAnti-Calcium-Binding Protein Erc-55 antibodyAnti-E6-Binding Protein antibodyAnti-E6bp antibodyAnti-RCN2 antibodyAnti-ERC55 antibody |
Information sourced from Uniprot.org