• Western blot analysis of extracts of various cell lines, using RBM22 antibody (STJ112065) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of Raji cells, using RBM22 antibody (STJ112065) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-RBM22 antibody (1-230) (STJ112065)

SKU:
STJ112065

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RBM22 (1-230) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RBM22
Gene ID: 55696
Uniprot ID: RBM22_HUMAN
Immunogen Region: 1-230
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human RBM22 (NP_060517.1).
Immunogen Sequence: MATSLGSNTYNRQNWEDADF PILCQTCLGENPYIRMTKEK YGKECKICARPFTVFRWCPG VRMRFKKTEVCQTCSKLKNV CQTCLLDLEYGLPIQVRDAG LSFKDDMPKSDVNKEYYTQN MEREISNSDGTRPVGMLGKA TSTSDMLLKLARTTPYYKRN RPHICSFWVKGECKRGEECP YRHEKPTDPDDPLADQNIKD RYYGINDPVADKLLKRASTM PRLDPPEDKT
Function Required for pre-mRNA splicing as component of the activated spliceosome. Involved in the first step of pre-mRNA splicing. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Involved in both translocations of the nuclear SLU7 to the cytoplasm and the cytosolic calcium-binding protein PDCD6 to the nucleus upon cellular stress responses.
Protein Name Pre-Mrna-Splicing Factor Rbm22
Rna-Binding Motif Protein 22
Zinc Finger Ccch Domain-Containing Protein 16
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Cytoplasm
Nearly Exclusively Nuclear
Translocated From The Nucleus To The Cytoplasm After Heat Shock Cell Treatment
May Be Shuttling Between The Nucleus And The Cytosol
Alternative Antibody Names Anti-Pre-Mrna-Splicing Factor Rbm22 antibody
Anti-Rna-Binding Motif Protein 22 antibody
Anti-Zinc Finger Ccch Domain-Containing Protein 16 antibody
Anti-RBM22 antibody
Anti-ZC3H16 antibody
Anti-199G4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance