Anti-RAPGEF1 antibody [ZN140] (STJA0033379)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenFull length human recombinant protein of human RAPGEF1 (NP_941372) produced in HEK293T cell.
General Information
| Short Description | Mouse monoclonal anti-RAPGEF1 for use in IHC-P in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | ZN140 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 0.5-1.0 mg/mL |
| Purification | Affinity Chromatography |
| Dilution Range | IHC 1:50-1:100 |
| Formulation | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | RAPGEF1 |
| Gene ID | 2889 |
| Uniprot ID | RPGF1_HUMAN |
| Immunogen | Full length human recombinant protein of human RAPGEF1 (NP_941372) produced in HEK293T cell. |
Additional Info
| Tissue Specificity | Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver. |
| Post Translational Modifications | Phosphorylation at Tyr-504 enhances activity as Rap guanine nucleotide exchange factor. |
| Function | Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth. |
| Protein Name | Rap Guanine Nucleotide Exchange Factor 1Crk Sh3-Binding GnrpGuanine Nucleotide-Releasing Factor 2Protein C3g |
| Database Links | Reactome: R-HSA-170968Reactome: R-HSA-186763Reactome: R-HSA-8875555Reactome: R-HSA-9013423Reactome: R-HSA-9027284Reactome: R-HSA-912631 |
| Cellular Localisation | Early Endosome |
| Alternative Antibody Names | Anti-Rap Guanine Nucleotide Exchange Factor 1 antibodyAnti-Crk Sh3-Binding Gnrp antibodyAnti-Guanine Nucleotide-Releasing Factor 2 antibodyAnti-Protein C3g antibodyAnti-RAPGEF1 antibodyAnti-GRF2 antibody |
Information sourced from Uniprot.org